Core--Biomarker laboratory

为了支持超级基金计划，我们建议建立一个Biomarker实验室核心，该核心实验室将结合公共卫生学院、加州大学伯克利分校、加州大学旧金山分校分子流行病学实验室和儿童医院奥克兰研究所的资源和设施。新的Biomarker Core的主要目标是使用已建立的分子和细胞遗传学分析方法促进流行病学项目1-5样本的收集、处理和分析，并确保对这些样本进行适当的处理、储存、储存和测试。该核心将参与这些标本的处理、存储、入库和测试。该核心将参与从不同种族背景的儿童白血病病例和健康对照(项目1-2)、印度和/或智利接触砷的儿童和成人(项目3)以及中国接触苯的工人(项目4-5)获取标本。将为脐带血、外周血、口腔和尿路上皮细胞以及精子样本建立复杂样本处理的实验室方案，包括超低温保存和液氮储存。这些生物样本的常规鉴定将包括DNA/RNA的分离和储存；N-RAS基因的突变分析；一组癌症易感基因的基因分型；通过逆转录聚合酶链式反应和荧光原位杂交鉴定儿童白血病病例的细胞遗传学特征；以及通过微核试验分析不同细胞类型的细胞遗传学损伤。将创建一个计算机数据库，以管理整个核心和超级基金计划中的样品跟踪、回收和传输。这些服务将大大提高流行病学项目#1-5实现其目标的能力。建立用于实验室分析的生物标记物核心是对Superfund计划资源的有效利用，因为UCB、UCSF和CHORI的合作者已经能够执行所需的所有分析，并且可以在不产生外部实验室费用的情况下这样做。将建立一个独特的组织和DNA/RNA样本库，来自不同种族背景的儿童和成人，用于未来因遗传和环境因素而产生的癌症风险。

In order to support the Superfund Program, we propose to establish a Biomarker laboratory Core that will combine the resources and facilities at the School of Public Health, UC Berkeley, the Laboratory of Molecular Epidemiology, UCSF, and Children's Hospital Oakland Research Institute. The main goal of the new Biomarker Core is to facilitate the collection, processing, and analysis of samples for epidemiological Projects 1-5 using established molecular and cytogenetic methods of analysis and to assure appropriate handling, storing, banking, and testing of these specimens. This core will be involved in the acquisition of handling, storing, banking, and testing of these specimens. This core will be involved in the acquisition of specimens from childhood leukemia cases and healthy controls of different ethnic backgrounds (with Projects #1-2); arsenic exposed children and adults from India and/or Chile (with Project #3); and benzene exposed workers from China (with Projects #4- 5). Laboratory protocols for sophisticated sample processing, including cryopreservation and storage in liquid nitrogen, will be established for cord blood, peripheral blood, buccal and urothelial cells, and sperm specimens. Routine characterization of these biological samples will include DNA/RNA isolation and storage; mutational analysis of the N- RAS gene; genotyping of a panel of cancer susceptibility genes; characterization of the cytogenetic traits in childhood leukemia cases by reverse-transcriptase PCR and fluorescence in situ hybridization; and analysis of cytogenetic damage in different cell types by the micronucleus assay. A computer database will be created to manage sample tracking, recovery, and transmission throughout the core and the Superfund program. These services will greatly enhance the ability of the epidemiological Projects #1-5 to achieve their goals. Establishing a biomarker core for laboratory analysis is an efficient use of resources of the Superfund program as UCB, UCSF and CHORI collaborators are already capable of performing all of the assays required and can do so without incurring the expenses of an outside laboratory. A unique bank of tissues and DNA/RNA samples will be established from children and adults of different ethnic background for use in future of cancer risk from genetic and environmental factors.