Core--Biomarker laboratory

为了支持超级基金计划，我们建议建立一个生物标志物实验室核心，该实验室将联合收割机的资源和设施在公共卫生学院，加州大学伯克利分校，分子流行病学实验室，加州大学旧金山分校，和儿童医院奥克兰研究所。新生物标志物核心的主要目标是使用已建立的分子和细胞遗传学分析方法促进流行病学项目1-5的样本收集、处理和分析，并确保这些标本的适当处理、储存、储存和检测。该核心将参与这些标本的处理、储存、储存和检测。该核心将参与采集来自不同种族背景的儿童白血病病例和健康对照的样本（项目1 -2）;来自印度和/或智利的砷暴露儿童和成人（项目3）;以及来自中国的苯暴露工人（项目4 - 5）。将为脐带血、外周血、口腔和尿路上皮细胞以及精子标本建立复杂样本处理的实验室方案，包括冷冻保存和液氮储存。这些生物样本的常规表征将包括DNA/RNA分离和储存; N-RAS基因的突变分析;一组癌症易感基因的基因分型;通过逆转录酶PCR和荧光原位杂交表征儿童白血病病例的细胞遗传学特征;以及通过微核试验分析不同细胞类型的细胞遗传学损伤。将建立一个计算机数据库，以管理整个核心和超级基金计划的样本跟踪、回收和传输。这些服务将大大提高流行病学项目1 -5实现其目标的能力。为实验室分析建立一个生物标志物核心是对超级基金项目资源的有效利用，因为UCB、UCSF和CHORI的合作者已经能够进行所有所需的测定，并且可以在不产生外部实验室费用的情况下进行。将从不同种族背景的儿童和成人中建立一个独特的组织和DNA/RNA样本库，用于未来遗传和环境因素引起的癌症风险。

In order to support the Superfund Program, we propose to establish a Biomarker laboratory Core that will combine the resources and facilities at the School of Public Health, UC Berkeley, the Laboratory of Molecular Epidemiology, UCSF, and Children's Hospital Oakland Research Institute. The main goal of the new Biomarker Core is to facilitate the collection, processing, and analysis of samples for epidemiological Projects 1-5 using established molecular and cytogenetic methods of analysis and to assure appropriate handling, storing, banking, and testing of these specimens. This core will be involved in the acquisition of handling, storing, banking, and testing of these specimens. This core will be involved in the acquisition of specimens from childhood leukemia cases and healthy controls of different ethnic backgrounds (with Projects #1-2); arsenic exposed children and adults from India and/or Chile (with Project #3); and benzene exposed workers from China (with Projects #4- 5). Laboratory protocols for sophisticated sample processing, including cryopreservation and storage in liquid nitrogen, will be established for cord blood, peripheral blood, buccal and urothelial cells, and sperm specimens. Routine characterization of these biological samples will include DNA/RNA isolation and storage; mutational analysis of the N- RAS gene; genotyping of a panel of cancer susceptibility genes; characterization of the cytogenetic traits in childhood leukemia cases by reverse-transcriptase PCR and fluorescence in situ hybridization; and analysis of cytogenetic damage in different cell types by the micronucleus assay. A computer database will be created to manage sample tracking, recovery, and transmission throughout the core and the Superfund program. These services will greatly enhance the ability of the epidemiological Projects #1-5 to achieve their goals. Establishing a biomarker core for laboratory analysis is an efficient use of resources of the Superfund program as UCB, UCSF and CHORI collaborators are already capable of performing all of the assays required and can do so without incurring the expenses of an outside laboratory. A unique bank of tissues and DNA/RNA samples will be established from children and adults of different ethnic background for use in future of cancer risk from genetic and environmental factors.