Rapid miniaturized sensors for the dectection of environmental toxins

用于检测环境毒素的快速小型化传感器

• 批准号: 6333684
• 负责人: IAN Manning KENNEDY
• 金额: $ 16.52万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-30 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6333684
• 关键词: bioassay; bioengineering /biomedical engineering; biosensor device; environmental toxicology; hazardous substances; method development; miniature biomedical equipment; toxicant screening

The Superfund program at University of California Davis has produced assays for many environmentally hazardous materials. The application of these bioassays in fast, multi-analyte instruments requires further work that will be undertaken in the Biosensor projects. Initially, engineering of surface microdot systems will be carried out for model assays for atrazine and metabolites. Small dots of antibodies will be printed onto a surface and then exposed to samples. The sensitivity of the surface microdot system will be explored on the model assay. Automation of steps in cell based assays will also be investigated. Novel fluorescence labels will be developed in collaboration with other projects in the program. Three classes of new labels will be tested; they include porphyrins and lanthanides that are amenable to time resolved fluorescence detection. Fluorescence resonance energy transfer (FRET) will be investigated as a promising label. Experiments will be carried out on simple solutions and on a model assay for atrazine to evaluate sensitivity and detection limits. Microdroplets will be investigated a novel format for fluorescence detection. Micron sized droplets of sample with be interrogated with a laser. The possibility of using non linear optical processes, such as droplet lasing, in the microdroplets will be determined. Microfabrication technology will be used to design and fabricate miniaturized systems for immunoassays. Sample handling and processing will be incorporated into the miniaturized device. Ultrasonic agitation will be investigated as a way to improve binding rates. Finally, the miniaturized immunoassay system will be interfaced with novel detection methods, using new fluorophores and new detection formats (such as microdroplets). The sensitivity, detection limits, and turn around time of the packaged system, will be evaluated on atrazine and other compounds of interest, including dioxin and human reproductive hormones.

加州大学戴维斯分校的超级基金项目已经对许多环境有害物质进行了分析。这些生物分析在快速、多分析仪器中的应用需要在生物传感器项目中进行进一步的工作。最初，将对阿特拉津和代谢物的模型分析进行表面微点系统的工程设计。抗体的小斑点将被打印在表面上，然后暴露在样本中。表面微点系统的灵敏度将在模型试验中进行探索。基于细胞的分析步骤的自动化也将被研究。将与该计划中的其他项目合作开发新的荧光标签。将测试三类新标签；它们包括能够进行时间分辨荧光检测的卟啉和稀土元素。荧光共振能量转移(FRET)作为一种很有前途的标记物将被研究。实验将在简单的溶液和阿特拉津的模型化验上进行，以评估灵敏度和检测限。微滴将成为一种新的荧光检测方式。微米大小的样品液滴可以用激光探测。将确定在微滴中使用非线性光学过程的可能性，例如液滴激光。微制造技术将被用于设计和制造免疫分析的微型化系统。样品处理和处理将被整合到微型设备中。超声搅拌将作为提高结合率的一种方法进行研究。最后，小型化的免疫分析系统将与新的检测方法对接，使用新的荧光团和新的检测形式(如微滴)。包装系统的灵敏度、检测下限和周转时间将在阿特拉津和其他感兴趣的化合物上进行评估，包括二恶英和人类生殖激素。