Enhanced Liquid Core Waveguide Biosensor for PBDE Detection

用于 PBDE 检测的增强型液芯波导生物传感器

• 批准号: 7910125
• 负责人: IAN Manning KENNEDY
• 金额: $ 10.77万
• 依托单位: SYNTHIA, LLC
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7910125
• 关键词: 2-cyclopentyl-5-(5-isoquinolylsulfonyl)-6-nitro-1H-benzo(D)imidazole; Adverse effects; American; Antibodies; Back; Bacteriophage M13; Bacteriophages; Binding; Biological; Biological Assay; Biosensor; California; Carpet; Cells; Chemicals; Child; Complex; Coupled; Detection; Development; Device Designs; Devices; Drug Formulations; Dust; Dyes; Early Diagnosis; Electronics; Endocrine Disruptors; Energy Transfer; Environment; Environmental Exposure; Environmental Monitoring; Environmental Pollution; Environmental sludge; Enzyme-Linked Immunosorbent Assay; Exhibits; Exposure to; Female of child bearing age; Fire - disasters; Flame Retardants; Fluorescence Resonance Energy Transfer; Furniture; Goals; Haptens; Health; Home environment; Horseradish Peroxidase; House Dust; Human; Human Milk; Human Resources; Immunoassay; Individual; Ingestion; Label; Laboratories; Laboratory Animals; Lanthanoid Series Elements; Legal patent; Liquid substance; Marketing; Mattresses; Measurement; Measures; Methods; Metric; Molecular Weight; Monitor; Names; Nature; Neurologic; Pentas; Peptides; Performance; Phage Display; Phase; Plastics; Poison; Police; Polychlorinated Biphenyls; Preparation; Principal Investigator; Production; Property; Public Health; Reading; Recycling; Risk; Route; Sampling; Scheme; Screening procedure; Serum; Sewage; Signal Transduction; Site; System; Technology; Testing; Textiles; Thyroid Hormones; Time; Toxic effect; Toy; Training; Universities; Work; base; bioaccumulation; consumer product; cost effective; density; detector; dibenzo(1,4)dioxin; experience; exposed human population; innovation; instrument; meetings; particle; phenyl ether; polychlorinated dibenzofurans; polyurethane foam; public health relevance; sensor

DESCRIPTION (provided by applicant): Polybrominated diphenyl ethers (PBDEs) are common flame retardants used to reduce the risk of fire in a wide variety of products, such as mattresses, furniture, home electronics, textiles, upholstery fabrics, carpet, and children's toys. As a consequence, wide spread occurrence in the environment and bioaccumulation through various exposure routes have been revealed posing concerns to public health because of their potential adverse effects on human health. In this respect, there is a high demand to develop a field-portable rapid "on-site" detection method for early diagnosis of human exposure or environmental monitoring. In order to meet this need, Synthia-LLC proposes the development of a field-portable biosensor for BDE-47, one of the most toxic PBDE congeners. Using patented technology referred to as a phage anti-immunocomplex assay (PHAIA) which is a versatile non-competitive two site assay format for small compounds based on an analyte-antibody complex specific phage-borne peptide as the key technology, Synthia-LLC will develop a homogeneous fluorescent energy transfer PHAIA in a liquid core waveguide (FRET PHAIA-LCW) by setting three aims to achieve the goal. The first aim is the development of a standard 386-well plate based homogeneous FRET PHAIA by taking advantage of the poly binding nature of the phage particle. We expect that efficient fluorescent energy transfer through multiple interactions between an individual phage particle conjugated with the lanthanide donor in high density and many antibodies labeled with acceptor dye will not only enhance signal intensity, resulting in better sensitivity, but shorten general assay time because there is no need for repeated washing and incubation. The second aim is the establishment of a simple sample extraction method from biological samples. We expect that the simple extraction method will eliminate any matrix effect that may cause a false positive or negative result and thus enhance the detection accuracy of the assay. The third aim is a feasibility test of the FRET PHAIA-LCW in a simple device design. For Phase I, this test will be carried out using a commercially available liquid core waveguide cell. We expect that the property of the LCW in guiding the fluorescent signals to the detector with minimal signal loss will further enhance the assay sensitivity and the liquid phase assay can be easily extended to an automated compact type device. PUBLIC HEALTH RELEVANCE: The assay device proposed by Synthia-LLC will ultimately be a rapid, field portable device that can be used to provide precise measures of environmental exposure of humans to polybrominated diphenyl ether flame retardants (PBDEs). Precise measurement information can then be used to reduce exposures and thus risk of these toxic compounds.

描述（由申请人提供）：多溴联苯醚（PBDEs）是一种常见的阻燃剂，用于降低各种产品的火灾风险，如床垫、家具、家用电子产品、纺织品、室内装饰织物、地毯和儿童玩具。因此，已发现在环境中广泛存在并通过各种接触途径进行生物积累，对公共卫生构成关切，因为它们对人类健康可能产生不利影响。在这方面，迫切需要发展一种现场便携式快速“现场”检测方法，以便早期诊断人类接触或进行环境监测。为了满足这一需求，syntia - llc公司提出了一种用于BDE-47的野外便携式生物传感器的开发，BDE-47是毒性最大的PBDE同系物之一。利用噬菌体抗免疫复合物测定（PHAIA）的专利技术，这是一种基于分析抗体复合物特异性噬菌体携带肽的小化合物的通用非竞争性双位点测定格式，syntia - llc将通过设定三个目标来实现液体核心波导中的均匀荧光能量转移PHAIA （FRET PHAIA- lcw）。第一个目标是利用噬菌体颗粒的多结合特性，开发一种基于标准386孔板的均匀FRET PHAIA。我们期望通过高密度与镧系供体偶联的单个噬菌体颗粒与许多用受体染料标记的抗体之间的多重相互作用进行有效的荧光能量转移，不仅可以增强信号强度，从而提高灵敏度，而且可以缩短一般的检测时间，因为不需要重复洗涤和孵育。第二个目的是从生物样品中建立一种简单的样品提取方法。我们期望简单的提取方法能够消除任何可能导致假阳性或阴性结果的基质效应，从而提高检测的准确性。第三个目标是在一个简单的器件设计中对FRET PHAIA-LCW进行可行性测试。对于第一阶段，该测试将使用市售的液芯波导电池进行。我们期望LCW以最小的信号损失将荧光信号引导到检测器的特性将进一步提高分析灵敏度，并且液相分析可以很容易地扩展到自动化紧密型设备。