CYTOSKELETAL CELL SURFACE INTERACTION IN ORAL EPITHELIAL CELLS

这项研究的目标是确定基本的 调节细胞骨架间相互作用的机制 角蛋白中间丝与细胞表面的结合 牙龈的粘连连接、桥粒和半桥粒 上皮细胞。拟议研究的长期目标是 深入了解上皮细胞的分子基础 代表牙周病特征的迁移。这个 具体目标包括确定中间纤维如何 相关蛋白IFAP300连接桥粒和 半桥粒到中间花丝。为此，IFAP300 将被克隆和测序。一旦完整的cdna序列 如果有，将使用瞬时转染法来确定 这种重要的交叉桥蛋白的生理功能。 其他目的是在体外确定分子间的相互作用。 在角蛋白中，IFAP300和主要的细胞质斑块 桥粒(桥粒蛋白)和半桥粒的蛋白质 (BP230)。体内研究的目标将是确定 活细胞中角蛋白中间丝的动态特性 使用显微成像技术。

The objectives of this research are to determine the basic mechanisms that regulate the interactions between cytoskeletal keratin intermediate filaments and the cell surface associated adhesion junctions, desmosomes and hemidesmosomes, of gingival epithelia cells. The long term goal of the proposed studies is to gain insights into the molecular bases of the epithelial cell migration that represents a hallmark of periodontal disease. The specific aims include determining how the intermediate filament associated protein, IFAP300, connects desmosomes and hemidesmosomes to intermediate filaments. To this end, IFAP300 will be cloned and sequenced. Once the complete cDNA sequence is available, transient transfection will be used to determine the physiological functions of this important cross-bridging protein. Other aims are to determine in vitro the molecular interactions among keratins, IFAP300 and the major cytoplasmic plaque proteins of the desmosome (desmoplakin) and the hemidesmosome (BP230). In vivo studies will be targeted at determining the dynamic properties of keratin-intermediate filaments in living cells using microscopic imaging techniques.