AMINO ACID SEQ OF NEW ALLERGENIC ALBUMIN STORAGE PROTEIN FROM RICINUS COMMUNIS

来自蓖麻的新过敏性白蛋白储存蛋白的氨基酸序列

• 批准号: 6307612
• 负责人: LEWIS J GREENE
• 金额: $ 0.82万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-12-01 至 2000-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6307612
• 关键词: biomedical resource; genome; immunology; inflammation; plants; spectrometry

The isolation and sequence determination of a new 2S albumin storage protein from Ricinus communis seeds is described. The fragment approach using selective enzymatic cleavage, Edman degradation and mass spectrometry was used to demonstrate primary structure of the 11.3 kDa heterodimer protein which is linked by 4 disulfide bridges. Mass spectrometry (MALDI-TOF and ESI/MS) were used to obtain the molecular weight of the protein and its two constituent chains as a criterion for validating the sequence of each chain obtained by wet chemistry. The sequence of the amino terminal blocked peptide <Gln Gln Gln Glu was determined by ESI/MS which was impossible to do by wet chemistry. The characterization of the second 2S albumin, from R communis seeds demonstrates that the 237 residue precursor protein postulated on the basis on cDNA data codes for two different heterodimer proteins containing approximately 97 residues. A manuscript describing this work has been published J.G odinho da Silva, Jr., O.L.T. Machado, C. Izumi, J.C. Padovan, B.T. Chait, U.A. Mirza & L.J. Greene "Amino acid sequence of a new allergenic 2S albumin storage protein from Ricinus communis which is part of a 30 kDa precursor protein" Archives of Biochem. & Biophys. 336 (1996) 10-18.

一种新的2S白蛋白的分离和序列测定 对蓖麻籽中的贮藏蛋白进行了研究。这个 使用选择性酶切割的片段方法，埃德曼 降解和质谱学用于初步验证 4连接的11.3 kDa异源二聚体的结构 二硫键。使用质谱仪(MALDI-TOF和ESI/MS) 要获得蛋白质及其两个组分的相对分子质量 链作为验证每条链序列的标准 通过湿化学获得的。氨基末端的序列被阻断 用电喷雾质谱联用法测定多肽&谷氨酰胺不可能 通过湿化学反应来做。第二个2S的刻画 芦荟种子中的白蛋白表明，237个残基 根据cdna数据推测的前体蛋白 含有约97个残基的不同异源二聚体蛋白。 描述这项工作的手稿已发表在J.G.奥迪尼奥·达 首页--期刊主要分类--期刊细介绍--期刊题录与文摘--期刊详细文摘内容 米孜和L.J.格林“一种新的过敏原2S的氨基酸序列 来自蓖麻的白蛋白储存蛋白，它是30 《KDA前体蛋白》《生物化学与生物物理资料》336(1996) 10比18。