MASS SPECTROMETRIC ANALYSIS OF PROBLEM AMINO ACID SEQUENCES

许多蛋白质的氨基酸序列无法通过测序进行序列分析。 常规Edman测序程序。 困难出现了，因为 例如，通过阻塞或其他修改，以及通过 重复氨基酸残基。 我们用质谱仪 方法如碰撞诱导解离串联质谱 光谱法来从这些难处理的蛋白质中获得序列。 一 描述这项工作的手稿已经出版J.Godinho da Silva， 小的，O.L. T马查多角泉，J.C. Padovan，B.T. Chait，U.A.米尔扎& L. J.格林“一种新的致敏性2S白蛋白的氨基酸序列 来自蓖麻的储存蛋白，其是30 kDa的 生物化学与生物物理学文献。 336（1996）10-18。

Many protein amino acid sequences defy sequence analysis by the convention Edman Sequencing procedure. The difficulties arise, for example, through blocking or other modifications and through repetitive amino acid residues. We are using mass spectrometric methods such as collision-induced dissociation tandem mass spectrometry to obtain sequences from such intractable proteins. A manuscript describing this work has been published J.Godinho da Silva, Jr., O.L.T. Machado, C. Izumi, J.C. Padovan, B.T. Chait, U.A. Mirza & L.J. Greene "Amino acid sequence of a new allergenic 2S albumin storage protein from Ricinus communis which is part of a 30 kDa precursor protein" Archives of Biochem. & Biophys. 336 (1996) 10-18.