MASS SPECTROMETRIC ANALYSIS OF PROBLEM AMINO ACID SEQUENCES

问题氨基酸序列的质谱分析

• 批准号: 6279461
• 负责人: LEWIS J GREENE
• 金额: --
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-12-01 至 1998-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6279461
• 关键词: biological products; biomedical resource; proteins; spectrometry

Many protein amino acid sequences defy sequence analysis by the convention Edman Sequencing procedure. The difficulties arise, for example, through blocking or other modifications and through repetitive amino acid residues. We are using mass spectrometric methods such as collision-induced dissociation tandem mass spectrometry to obtain sequences from such intractable proteins. A manuscript describing this work has been published J.Godinho da Silva, Jr., O.L.T. Machado, C. Izumi, J.C. Padovan, B.T. Chait, U.A. Mirza & L.J. Greene "Amino acid sequence of a new allergenic 2S albumin storage protein from Ricinus communis which is part of a 30 kDa precursor protein" Archives of Biochem. & Biophys. 336 (1996) 10-18.

许多蛋白质氨基酸序列无法通过序列分析