Biomolecular Recognition Using Nanoparticle Receptors

使用纳米颗粒受体进行生物分子识别

• 批准号: 6320217
• 负责人: VINCENT M. ROTELLO
• 金额: $ 24.07万
• 依托单位: UNIVERSITY OF MASSACHUSETTS AMHERST
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-06-01 至 2004-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6320217
• 关键词: bioengineering /biomedical engineering; biomaterial development /preparation; biosensor device; molecular assembly /self assembly; nanotechnology; receptor

DESCRIPTION: (Applicant's Description) Biomolecular Recognition Using Self-Optimizing Mu1tivalentNanoparticle Receptors Specific recognition of biomolecular Systems is a fundamental goal inbiomedical research. The ability to create efficient receptors for biomolecules allows us to fabricate biosensors that allow real-time monitoring central to the rapid diagnosis of imbalances and illnesses. Recognition of biomacromolecules, including proteins, polysaccharides, and nucleic acids, extends our ability to create diagnostic devices, while also providing an important tool for the modulation of cellularprocesses. To provide a general route for the creation of receptors for small molecules and macromolecules, we have created hosts based on nanoparticle scaffolds. These hosts are readily fabricated from self-assembled Monolayer-Protected Clusters (MPCs), either through direct functionalization during particle formation, or via subsequent place exchangereactions provide Mixed Monolayer Protected Clusters (MMPCs). In preliminary studies, we have demonstrated the ability of MMPCs to efficiently recognize both small molecules and macromolecules. Significantly, these receptors are dynamic, and can be templated through non-covalent interactions. In our proposed research, we will explore the fundamental aspects of these self-optimizing nanoparticle-based receptors, including the effect of monolayer structure, headgroups, and crosslinking on target recognition. Concurrently, we will apply these receptors to the recognition of guests possessing multiple size scales, from small molecule guests to peptides and protein surfaces. We will also explore the recognition of cellular structures, and the use of this recognition for both imaging and therapeutic applications.

描述：（申请人的描述）使用生物分子识别 自优化多价纳米粒子受体 对生物分子系统的特异性识别是生物医学的基本目标 研究。为生物分子创建有效受体的能力使我们能够 制造能够实时监控快速反应的生物传感器 失衡和疾病的诊断。生物大分子的识别， 包括蛋白质、多糖和核酸，扩展了我们的能力 创建诊断设备，同时也为诊断提供重要工具 细胞过程的调节。 提供创建小分子受体的一般途径 和大分子，我们创建了基于纳米颗粒支架的宿主。 这些主机很容易由自组装的单层保护材料制成 簇（MPC），通过粒子过程中的直接功能化 形成，或通过随后的交换反应提供混合单层 受保护的集群 (MMPC)。在初步研究中，我们已经证明了 MMPCs 有效识别小分子和 大分子。值得注意的是，这些受体是动态的，并且可以 通过非共价相互作用模板化。在我们提出的研究中，我们将 探索这些基于纳米粒子的自我优化的基本方面 受体，包括单层结构、头基和 目标识别上的交联。同时，我们将应用这些受体 到对拥有多种尺寸尺度的客人的认可，从小到大 分子客体来到肽和蛋白质表面。我们还将探索 细胞结构的识别，以及这种识别的用途 成像和治疗应用。