Synthesis and Study of Novel Sensing Agents

新型传感剂的合成与研究

• 批准号: 6332294
• 负责人: ROBERT M STRONGIN
• 金额: $ 18.38万
• 依托单位: LOUISIANA STATE UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-05-01 至 2006-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6332294
• 关键词: chemical synthesis; colorimetry; diagnosis design /evaluation; diagnostic tests; fructose; oligosaccharides; reagent /indicator; sialate; technology /technique development

DESCRIPTION: (Applicant's Description) This proposal addresses three important problem in biomedical science via the development of simple and rapid assays for biomolecules. The first goal involves the development of a room temperature color assay for fructose, a ubiquitous, rapidly metabolized sugar. The proposedassay is designed to be fructose-selective in the presence of a large excess of glucose. Fructose determination in plasma is currently hampered due to the large excess of glucose. The second goal involves creatinga mild assay for sialic acid, an important cell surface antigen, to address the problem of current assay inaccuracy based on hydrolytic procedures and/or relatively non-specific and harsh methods. The third goal entails the implementation of a new automated color detection system for oligosaccharides that does not require prior destructive hydrolysis or chemical labeling with a chromophore. Currently no detection technique exists for neutral oligosaccharides that is effective for the visual detection of oligosaccharides beyond three repeat units.

描述：(申请人的描述)这份提案涉及三个重要的方面 通过开发简单快速的检测方法解决生物医学中的问题 为了生物分子。第一个目标涉及室温的发展。 果糖的比色分析，果糖是一种普遍存在的快速代谢的糖。这个 所提出的检测方法设计为在存在大量果糖的情况下对果糖具有选择性 葡萄糖过多。血浆中果糖的测定目前因 大量过量的葡萄糖。第二个目标是创建一种温和的化验方法 对于唾液酸，一种重要的细胞表面抗原，以解决 目前的化验不准确基于水解法和/或相对 不具体、严苛的做法。第三个目标需要实施一个 一种新的寡糖自动颜色检测系统，不需要 先前的破坏性水解或用生色团进行化学标记。目前 目前还不存在对中性寡糖有效的检测技术 用于超过三个重复单元的低聚糖的目视检测。