权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

A Simple and Robust Indicator for Glutathione

简单而可靠的谷胱甘肽指标

基本信息

批准号：
8627059
负责人：
ROBERT M STRONGIN
金额：
$ 43.46万
依托单位：
PORTLAND STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-04-01 至 2017-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8627059
关键词：
Accounting Amino Acids Autistic Disorder Basic Science Binding Biohazardous Substance Biological Biological Assay Biological Markers Blood Blood specimen Cardiac Surgery procedures Cause of Death Cells Cessation of life Chemicals Chronic Disease Chronic Kidney Failure Clinical Research Data Detection Diagnosis Diagnostic tests Disease Disease Progression Erythrocytes Evaluation Evaluation Studies Exhibits Fingers Fluorescence Fluorescence Resonance Energy Transfer Generations Glutathione Goals Health Heart failure Heel Hemoglobin Human Knowledge Label Laboratories Life Liver diseases Lung diseases Malignant Neoplasms Measurement Medicine Methodology Methods Mitochondrial Diseases Monitor Optics Parkinson Disease Pathogenesis Patients Peritoneal Dialysis Physiological Plasma Play Postoperative Period Process Property Protons Reporting Research Research Personnel Role Running Sampling Signal Transduction Testing Therapeutic Intervention Time Variant Whole Blood Work analytical method base cost design flexibility fluorophore innovation interest minimally invasive oxidation point of care public health relevance receptor response simulation validation studies

项目摘要

Chronic diseases are the leading cause of death in the world, accounting for over 60 % of all fatalities. Decreased levels of the tripeptide glutathione (GSH) are well-known to be associated with chronic diseases and are of interest in monitoring disease progression and therapeutic interventions. For example, recent studies show that monitoring whole blood glutathione (GSH) levels can be of benefit towards diagnosing asymptomatic heart failure and mitochondrial disorders. GSH monitoring is also useful in the health assessment of patients with chronic renal disease or post-operative open heart surgery. However, reported levels of GSH in both healthy subjects and patients exhibit inter-laboratory variation. Plasma GSH levels are often reported despite the fact that they account for less than 0.5 % of the circulating GSH. Divergent sample processing and conjugation steps propagate inter-laboratory variability, as they result in varying degrees of sample oxidation and labeling. In order to achieve consistent and efficient GSH monitoring, the major goal of this proposal is to develop a fluorescent indicator that functions directly in minimally-diluted whole blood. In order to achieve this goal, the following specific aims are proposed: 1. Design, synthesis and evaluation of NIR-active GSH-selective indicators. Based on the preliminary data referred to above, our working hypothesis is that highly selective indicators for GSH can be designed to bind GSH via multipoint supramolecular interactions and produce concomitant fluorescence signals without optical interference from hemoglobin or other blood components. 2. Develop methodology for detection and quantitation of GSH in whole blood. We postulate, again on the basis of our preliminary data, that selectivity and sensitivity for GSH over a disease-relevant range can be achieved in human blood via the design of GSH-selective NIR active probe-containing receptors. The studies proposed in this aim are thus focused on optimization and demonstration of the utility of the proposed design principles and synthetic indicators via extensive evaluation and validation studies.

慢性病是世界上导致死亡的主要原因，占所有死亡人数的60%以上。

项目成果

期刊论文数量（9）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（1）

Recent progress in chromogenic and fluorogenic chemosensors for hypochlorous acid.

DOI：
10.1039/c6an00158k
发表时间：
2016-03-21
期刊：
The Analyst
影响因子：
0
作者：
Yue Y;Huo F;Yin C;Escobedo JO;Strongin RM
通讯作者：
Strongin RM

Varied Length Stokes Shift BODIPY-Based Fluorophores for Multicolor Microscopy.

DOI：
10.1038/s41598-018-22892-8
发表时间：
2018-03-15
期刊：
Scientific reports
影响因子：
4.6
作者：
Bittel AM;Davis AM;Wang L;Nederlof MA;Escobedo JO;Strongin RM;Gibbs SL
通讯作者：
Gibbs SL

Assessment of human pancreas cancer tissue and precursor lesions via a fluorophore with inherent PDAC selectivity.

通过具有固有 PDAC 选择性的荧光团评估人类胰腺癌组织和癌前病变。

DOI：
10.1016/j.ymeth.2019.06.008
发表时间：
2019
期刊：
Methods (San Diego, Calif.)
影响因子：
0
作者：
Munhenzva,IanR;Barth,ConnorW;Sibrian-Vazquez,Martha;Wang,LeiG;Escobedo,JorgeO;Gibbs,SummerL;Strongin,RobertM
通讯作者：
Strongin,RobertM

A simple assay for glutathione in whole blood.

DOI：
10.1039/c5an00345h
发表时间：
2015-05-21
期刊：
The Analyst
影响因子：
0
作者：
Hakuna L;Doughan B;Escobedo JO;Strongin RM
通讯作者：
Strongin RM

Far-Red and Near-Infrared Seminaphthofluorophores for Targeted Pancreatic Cancer Imaging.

DOI：
10.1021/acsomega.6b00403
发表时间：
2017-01-31
期刊：
ACS omega
影响因子：
4.1
作者：
Wang L;Barth CW;Sibrian-Vazquez M;Escobedo JO;Lowry M;Muschler J;Li H;Gibbs SL;Strongin RM
通讯作者：
Strongin RM