A Simple and Robust Indicator for Glutathione
A Simple and Robust Indicator for Glutathione
批准号:
8627059
负责人:
ROBERT M STRONGIN
金额:
$43.46万
依托单位国家:
美国
项目类别:
财政年份:
2014
资助国家:
美国
项目状态:
已结题
起止时间:
2014-04-01 至 2017-03-31
关键词:
AccountingAmino AcidsAutistic DisorderBasic ScienceBindingBiohazardous SubstanceBiologicalBiological AssayBiological MarkersBloodBlood specimenCardiac Surgery proceduresCause of DeathCellsCessation of lifeChemicalsChronic DiseaseChronic Kidney FailureClinical ResearchDataDetectionDiagnosisDiagnostic testsDiseaseDisease ProgressionErythrocytesEvaluationEvaluation StudiesExhibitsFingersFluorescenceFluorescence Resonance Energy TransferGenerationsGlutathioneGoalsHealthHeart failureHeelHemoglobinHumanKnowledgeLabelLaboratoriesLifeLiver diseasesLung diseasesMalignant NeoplasmsMeasurementMedicineMethodologyMethodsMitochondrial DiseasesMonitorOpticsParkinson DiseasePathogenesisPatientsPeritoneal DialysisPhysiologicalPlasmaPlayPostoperative PeriodProcessPropertyProtonsReportingResearchResearch PersonnelRoleRunningSamplingSignal TransductionTestingTherapeutic InterventionTimeVariantWhole BloodWorkanalytical methodbasecostdesignflexibilityfluorophoreinnovationinterestminimally invasiveoxidationpoint of carepublic health relevancereceptorresponsesimulationvalidation studies
中文摘要
慢性病是世界上导致死亡的主要原因,占所有死亡人数的60%以上。
英文摘要
Chronic diseases are the leading cause of death in the world, accounting for over 60 % of all fatalities.
Decreased levels of the tripeptide glutathione (GSH) are well-known to be associated with chronic
diseases and are of interest in monitoring disease progression and therapeutic interventions. For
example, recent studies show that monitoring whole blood glutathione (GSH) levels can be of benefit
towards diagnosing asymptomatic heart failure and mitochondrial disorders. GSH monitoring is also
useful in the health assessment of patients with chronic renal disease or post-operative open heart
surgery. However, reported levels of GSH in both healthy subjects and patients exhibit inter-laboratory
variation. Plasma GSH levels are often reported despite the fact that they account for less than 0.5 %
of the circulating GSH. Divergent sample processing and conjugation steps propagate inter-laboratory
variability, as they result in varying degrees of sample oxidation and labeling. In order to achieve
consistent and efficient GSH monitoring, the major goal of this proposal is to develop a fluorescent
indicator that functions directly in minimally-diluted whole blood. In order to achieve this goal, the
following specific aims are proposed:
1. Design, synthesis and evaluation of NIR-active GSH-selective indicators.
Based on the preliminary data referred to above, our working hypothesis is that highly selective
indicators for GSH can be designed to bind GSH via multipoint supramolecular interactions and
produce concomitant fluorescence signals without optical interference from hemoglobin or other
blood components.
2. Develop methodology for detection and quantitation of GSH in whole blood.
We postulate, again on the basis of our preliminary data, that selectivity and sensitivity for GSH
over a disease-relevant range can be achieved in human blood via the design of GSH-selective
NIR active probe-containing receptors. The studies proposed in this aim are thus focused on
optimization and demonstration of the utility of the proposed design principles and synthetic
indicators via extensive evaluation and validation studies.
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DOI:
10.1039/c6an00158k
发表时间:
2016-03-21
期刊:
The Analyst
影响因子:
--
作者:
[Yue Y, Huo F, Yin C, Escobedo JO, Strongin RM]
通讯作者:
Strongin RM
DOI:
10.1038/s41598-018-22892-8
发表时间:
2018-03-15
期刊:
Scientific reports
影响因子:
4.6
作者:
[Bittel AM, Davis AM, Wang L, Nederlof MA, Escobedo JO, Strongin RM, Gibbs SL]
通讯作者:
Gibbs SL
Assessment of human pancreas cancer tissue and precursor lesions via a fluorophore with inherent PDAC selectivity.
通过具有固有 PDAC 选择性的荧光团评估人类胰腺癌组织和癌前病变。
DOI:
10.1016/j.ymeth.2019.06.008
发表时间:
2019
期刊:
Methods (San Diego, Calif.)
影响因子:
--
作者:
[Munhenzva,IanR, Barth,ConnorW, Sibrian-Vazquez,Martha, Wang,LeiG, Escobedo,JorgeO, Gibbs,SummerL, Strongin,RobertM]
通讯作者:
Strongin,RobertM
DOI:
10.1021/acsomega.6b00403
发表时间:
2017-01-31
期刊:
ACS omega
影响因子:
4.1
作者:
[Wang L, Barth CW, Sibrian-Vazquez M, Escobedo JO, Lowry M, Muschler J, Li H, Gibbs SL, Strongin RM]
通讯作者:
Strongin RM
DOI:
10.1039/c5an00345h
发表时间:
2015-05-21
期刊:
The Analyst
影响因子:
--
作者:
[Hakuna L, Doughan B, Escobedo JO, Strongin RM]
通讯作者:
Strongin RM
共 8 条
Toxicant Production and Mitigation in the Electronic-Cigarette Reaction Vessel
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批准号:9560821
-
项目类别:
-
资助金额:$71.87万
-
财政年份:2015
-
负责人:ROBERT M STRONGIN
-
依托单位:
Toxicant Production and Mitigation in the Electronic-Cigarette Reaction Vessel
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批准号:9306920
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项目类别:
-
资助金额:$72.09万
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财政年份:2015
-
负责人:ROBERT M STRONGIN
-
依托单位:
Toxicant Production and Mitigation in the Electronic-Cigarette Reaction Vessel
-
批准号:8874702
-
项目类别:
-
资助金额:$68.69万
-
财政年份:2015
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负责人:ROBERT M STRONGIN
-
依托单位:
MBRS IMSD Program at Louisiana State University
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批准号:6863719
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项目类别:
-
资助金额:$17.13万
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财政年份:2004
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负责人:ROBERT M STRONGIN
-
依托单位:
MBRS IMSD Program
-
批准号:6711902
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项目类别:
-
资助金额:$17.43万
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财政年份:2004
-
负责人:ROBERT M STRONGIN
-
依托单位:
MBRS IMSD Program at Louisiana State University
-
批准号:7036580
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项目类别:
-
资助金额:$17.65万
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财政年份:2004
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负责人:ROBERT M STRONGIN
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依托单位:
SYNTHESIS OF INHIBITORS OF BIOTIN CARBOXYLASE
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批准号:6665891
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项目类别:
-
资助金额:$15.75万
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财政年份:2002
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负责人:ROBERT M STRONGIN
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依托单位:
Synthesis and Study of Novel Sensing Agents
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批准号:6332294
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项目类别:
-
资助金额:$18.38万
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财政年份:2001
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负责人:ROBERT M STRONGIN
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依托单位:
Synthesis and Study of Novel Sensing Agents
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批准号:6520327
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项目类别:
-
资助金额:$18.38万
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财政年份:2001
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负责人:ROBERT M STRONGIN
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依托单位:
Synthesis and Study of Novel Sensing Agents
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批准号:6747714
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项目类别:
-
资助金额:$18.38万
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财政年份:2001
-
负责人:ROBERT M STRONGIN
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依托单位:
Synthesis and Study of Novel Sensing Agents
-
批准号:6636510
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项目类别:
-
资助金额:$18.38万
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财政年份:2001
-
负责人:ROBERT M STRONGIN
-
依托单位:
Synthesis and Study of Novel Sensing Agents
-
批准号:7568898
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项目类别:
-
资助金额:$25.82万
-
财政年份:2001
-
负责人:ROBERT M STRONGIN
-
依托单位:
Synthesis and Study of Novel Sensing Agents
-
批准号:7264268
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项目类别:
-
资助金额:$26.25万
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财政年份:2001
-
负责人:ROBERT M STRONGIN
-
依托单位:
Synthesis and Study of Novel Sensing Agents
-
批准号:7795818
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项目类别:
-
资助金额:$25.58万
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财政年份:2001
-
负责人:ROBERT M STRONGIN
-
依托单位:
SYNTHESIS OF INHIBITORS OF BIOTIN CARBOXYLASE
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批准号:6486771
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项目类别:
-
资助金额:$15.75万
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财政年份:2001
-
负责人:ROBERT M STRONGIN
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依托单位:
Synthesis and Study of Novel Sensing Agents
-
批准号:6886743
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项目类别:
-
资助金额:$18.38万
-
财政年份:2001
-
负责人:ROBERT M STRONGIN
-
依托单位:
SYNTHESIS OF INHIBITORS OF BIOTIN CARBOXYLASE
-
批准号:6336841
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项目类别:
-
资助金额:$0.11万
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财政年份:2000
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负责人:ROBERT M STRONGIN
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依托单位:
海外基金