CHARACTERIZATION OF POLYCOMB GROUP COMPLEXES

Polycomb 基团复合物的表征

• 批准号: 6363353
• 负责人: ROBERT KINGSTON
• 金额: $ 20.76万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-03-01 至 2004-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6363353
• 关键词: DNA binding protein; Drosophilidae; Xenopus oocyte; chromatin; developmental genetics; gene induction /repression; homeobox genes; microarray technology; nucleosomes; protein sequence; suppressor mutations; transcription factor

DESCRIPTION (adapted from applicant's abstract): The polycomb-group (Pc-G) genes of Drosophila are required to maintain appropriate repression of genes that regulate developmental processes. Pc-G genes are conserved in mammals, and mutation of the mammalian homologs leads to severe developmental defects. It is believed that Pc-G proteins function, at least in part, by forming complexes that establish a repressive chromatin structure that maintains the 'off' state of homeotic genes. In order to investigate the mechanism of repression, it is necessary to purify Pc-G complexes and to establish assays that display the function of these complexes. This proposal describes an analysis of a Pc-G complex called PRC1 that has been isolated from Drosophila and that contains several Pc-G gene products. This analysis includes the following aims: 1) Identification of the proteins that make up PRC1. There are several unknown proteins in PRC1, and these proteins will be sequenced, their cDNAs will be isolated, and the encoded proteins will be characterized using genetic and biochemical approaches. 2) A detailed functional analysis of PRC1 will be performed. 3) The human homolog of PRC1 will be isolated and characterized. These experiments should increase the knowledge of how genes are maintained in a repressed state during development.

描述(改编自申请人的摘要)：多梳基团(PC-G) 果蝇的基因需要维持适当的基因抑制 来调节发育过程。PC-G基因在哺乳动物中是保守的， 哺乳动物同源基因的突变会导致严重的发育缺陷。它 人们认为Pc-G蛋白的功能至少部分是通过形成复合体来实现的 它建立了一种抑制染色质结构，以维持“关”的状态 同源异型基因。为了研究压抑的机制，它是 纯化Pc-G复合体和建立显示Pc-G复合体的分析所必需的 这些复合体的功能。这份提案描述了对PC-G的分析 一种从果蝇中分离出来的名为PRC1的复合体，含有 几种Pc-G基因产物。这一分析包括以下目的：1) 鉴定组成Prc1的蛋白质。有几个未知的 PRC1中的蛋白质，这些蛋白质将被测序，它们的cDNA将 分离出来，编码的蛋白质将用遗传和 生化方法。2)将对PRC1进行详细的功能分析 已执行。3)将分离和鉴定Prc1的人类同源基因。 这些实验应该会增加关于基因是如何在体内维持的知识 在发展中被压抑的状态。