ADAPTIVE REGULATION IN 3T3 L1 ADIPOCYTES

描述：碳水化合物调节基因提供了一种 将蜂窝响应与资源利用在整个 尸体。这一协调努力为维护 人体内循环中的葡萄糖水平在一个相当窄的范围内。 葡萄糖缺乏会增加葡萄糖的转运速度。 受体通过蛋白质合成依赖的机制在许多细胞中分型。 然而，GLUT1葡萄糖转运体的表达没有变化。 这项提议的重点是发现一种独特的50 kDa蛋白质，其 合成受葡萄糖供应的调节。这项研究建议测试 该50kDA蛋白调节GLUT 1活性的假说 来影响葡萄糖摄取的变化。这项研究计划涉及一系列 的特定目的来定义p50蛋白在 3T3-L1脂肪细胞转录和翻译水平的研究 老鼠。实验的目的是研究p50与 供应过剩！以及这种相互作用对运输活动的影响。最后， 建议研究p50在运输功能中的作用。 体内，使用反义技术抑制其表达和腺病毒 刺激过度表达的转导系统。

DESCRIPTION: Carbohydrate regulated genes provide a mechanism for integrating cellular responses with resource utilization in the entire body. This coordinated effort provides exquisite regulation in maintaining the circulating levels of glucose in humans within a fairly narrow range. Glucose deprivation increases the rate of glucose transport by the GLUT-1 receptor in a number cel types by a protein synthesis dependent mechanism. However, the expression of the GLUT1 glucose transporter does not change. This proposal focuses on the discovery of a unique 50 kDa protein whose synthesis is regulated by glucose availability. The studies proposed test the hypothesis that this 50 Kda protein modulates the activity of GLUT 1 to affect changes in glucose uptake. The research plan involves a series of specific aims which define the expression of the p50 protein at the transcriptional and translational level in 3T3-L1 adipocytes and the mouse. Experiments are designed to examine the interaction of p50 with GLUT! and the effect of that interaction on transport activity. Finally, studies are proposed to examine the role of p50 in transport function, in vivo, using antisense technology to inhibit it expression and adenovirus transduction system to stimulate overexpression.