Core--Nucleic Acid Synthesis and Sequencing

核心——核酸合成与测序

• 批准号: 6313609
• 负责人: HANSJUERG ALDER
• 金额: $ 15.13万
• 依托单位: THOMAS JEFFERSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-03-01 至 2005-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6313609
• 关键词: biomedical facility; genetic mapping; imaging /visualization /scanning; nucleic acid chemical synthesis; nucleic acid purification; nucleic acid sequence

DESCRIPTION: (Applicant's Description) Core B, the Nucleic Acid Synthesis and Sequencing Core is critical to the success of each of the projects. The Core, which is situated within the Sydney Kimmel Nucleic Acid Facility, is used on a daily basis by all of the Project leaders and their laboratory staff. Specifically for the Program, because it is involved in sequencing on the order of hundreds of kilobases, the Core will dedicate a full-time research assistant and a sequencing machine for the Program. We have found it an efficient mechanism for the heaviest users of the Facility to work directly with one technician under the supervision of Hannes Alder. Project 2 will require more than 3000 sequencing runs just to complete another 200 kilobases of sequence and not considering sequencing of the multiple inverse PCR products. Projects 1 and 3 will need regular sequencing runs also to characterize mutants, constructs, Fhit interacting gene candidates, genomic clones and PCR products. All the Projects will require numerous oligonucleotides, with Project 2 requiring on the order of 300 in the first year for characterization of the regions of FHIT remaining to be sequenced.

描述：（申请人的描述）核心B，核酸合成和测序核心是每个项目成功的关键。 位于Sydney Kimmel核酸设施内的核心，每天由所有项目负责人及其实验室工作人员使用。 特别是对于该计划，因为它涉及数百个酶的测序，核心将专门为该计划提供一名全职研究助理和一台测序机。我们发现，设施的最大用户在Hannes桤木的监督下直接与一名技术人员一起工作是一种有效的机制。 项目2将需要超过3000次测序运行，以完成另外200个测序，而不考虑多重反向PCR产物的测序。 项目1和3还需要定期测序运行来表征突变体、构建体、Fhit相互作用候选基因、基因组克隆和PCR产物。 所有项目都需要大量寡核苷酸，项目2在第一年需要大约300个寡核苷酸，用于表征剩余待测序的FHIT区域。