Core--Nucleic Acid Synthesis and Sequencing

核心——核酸合成与测序

• 批准号: 6570500
• 负责人: HANSJUERG ALDER
• 金额: $ 15.13万
• 依托单位: THOMAS JEFFERSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6570500
• 关键词: biomedical facility; genetic mapping; imaging /visualization /scanning; nucleic acid chemical synthesis; nucleic acid purification; nucleic acid sequence

DESCRIPTION: (Applicant's Description) Core B, the Nucleic Acid Synthesis and Sequencing Core is critical to the success of each of the projects. The Core, which is situated within the Sydney Kimmel Nucleic Acid Facility, is used on a daily basis by all of the Project leaders and their laboratory staff. Specifically for the Program, because it is involved in sequencing on the order of hundreds of kilobases, the Core will dedicate a full-time research assistant and a sequencing machine for the Program. We have found it an efficient mechanism for the heaviest users of the Facility to work directly with one technician under the supervision of Hannes Alder. Project 2 will require more than 3000 sequencing runs just to complete another 200 kilobases of sequence and not considering sequencing of the multiple inverse PCR products. Projects 1 and 3 will need regular sequencing runs also to characterize mutants, constructs, Fhit interacting gene candidates, genomic clones and PCR products. All the Projects will require numerous oligonucleotides, with Project 2 requiring on the order of 300 in the first year for characterization of the regions of FHIT remaining to be sequenced.

描述：(申请人描述)核心B，核酸合成 而核心测序对每个项目的成功都至关重要。这个 位于悉尼基梅尔核酸设施内的CORE是 由所有项目负责人和他们的实验室每天使用 工作人员。特别是对于该计划，因为它涉及到 数十万个基数的订单，内核将全职投入 研究助理和该计划的测序机。我们已经找到了 使设施最繁忙的用户能够直接工作的有效机制 有一名技术员在汉尼斯·阿尔德的监督下。项目2将 需要3000多次测序才能完成另外200千个碱基 不考虑多重反向聚合酶链式反应的测序 产品。项目1和3还需要定期排序运行，以 突变体，结构，FHIT相互作用基因候选，基因组 克隆和扩增产物。所有的项目都将需要大量的 寡核苷酸，第一个项目2需要300个数量级 对尚待测序的FHIT区域进行定性的年份。