CORE--FLOW CYTOMETRY

核心——流式细胞术

• 批准号: 6300565
• 负责人: Eric J Bernhard
• 金额: $ 18.3万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-12-17 至 2000-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6300565
• 关键词: biomedical facility; cell cycle; cell sorting; flow cytometry; neoplasm /cancer chemotherapy; neoplasm /cancer radiation therapy; radiation resistance; radiation sensitivity; synchronous cell division

This program project application has as its central theme the idea that the cell cycle may provide targets that could be manipulated to alter the therapeutic outcome in cancer treatment. Assessing the validity of the this idea is totally dependent on the adequacy of the conditions used for the experimental assays. The most critical aspect of this is the cell synchrony and flow cytometry. Because adequate flow cytometry is central to all five projects, the decision has been made to centralize all flow analysis and have the bulk of the flow carried out by the most experienced team using a single facility. This ensures that all projects have access to the highest quality service and that the conditions and the form of analysis used throughout the entire project will be comparable. The team leader of this core, Dr. Bernard, is the most experienced scientist is QA aspects of flow cytometry and in synchronization procedures. The costs associated with core related to service center cost changed by the Cancer Center at the University of Pennsylvania for running flow samples. Additional funds have been requested to purchase a microcomputer and software for data analysis. Our experience is that the time available on the Cancer Center's computer for data analysis of large flow runs is frequently the bottleneck that prevents more rapid experimental progress. Frequently, the design of future experiments is dependent on having the analysis completed on previous runs. In time is unavailable for data analysis after data collection, it often results in part of the laboratory standing idle until the analysis is complete. Access to out own computer and software will eliminate this bottleneck.

该计划项目应用程序是其中心主题的想法 细胞周期可能会提供可以操纵的目标以改变 癌症治疗中的治疗结果。评估有效性 这个想法完全取决于用于使用条件的适当性 实验测定。最关键的方面是细胞 同步和流式细胞仪。因为足够的流式细胞仪是中心的 对于所有五个项目，已经决定集中所有流程 分析并具有最有经验的最有经验的流量 团队使用单个设施。这确保所有项目都可以访问 为最高质量的服务以及条件和形式 在整个项目中使用的分析将是可比的。团队 该核心的负责人伯纳德博士是最有经验的科学家是质量检查 流式细胞仪和同步程序的各个方面。费用 与癌症有关的与服务中心成本相关的核心相关 宾夕法尼亚大学跑步样品的中心。 已要求额外的资金购买微型计算机和 用于数据分析的软件。我们的经验是可用的时间 癌症中心的计算机用于大量流量的数据分析是 经常阻止更快的实验进步的瓶颈。 通常，未来实验的设计取决于 分析在以前的运行中完成。随着时间的流逝，数据不可用 一次数据收集后的分析，通常会导致一部分实验室 闲置直到分析完成。访问自己的计算机 软件将消除这种瓶颈。