CORE--FLOW CYTOMETRY FACILITY

核心——流式细胞仪

• 批准号: 6989520
• 负责人: ALBERT D DONNENBERG
• 金额: $ 10.66万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-22 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6989520
• 关键词: biomedical facility; cell cycle; cell sorting; flow cytometry; neoplasm /cancer

The UPCI Flow Cytometry Facility moved to its new location at the Hillman Cancer Center Research Pavilion in September 2002. The new laboratory consists of a 200 ft 2 user laboratory (UserLab), and a 1000 ft 2 cell sorting laboratory. The Flow Cytometry Facility of the University of Pittsburgh Cancer Institute provides services to over 50 users within the UPCI and 20 non-UPCI users. These services fall into five categories: 1) Training users to operate the Facility's two user-dedicated analytical flow cytometers; 2) Performing high speed fluorescence activated cell sorting for investigators; 3) Insuring that the instruments in the facility are properly calibrated on a daily basis and advising users concerning the proper settings for their experiments; 4) Advising investigators on experimental design and data analysis and assisting in performance of complex multi-parameter experiments; and 5) Archiving all data produced by the facility. The current instrumentation at the UPCI Flow Cytometry Facility includes a Dako-Cytomation MoFLo high speed cell sorter, equipped with a CytoShield Biocontainment hood, a Dako-Cytomation CyAn 9-color high speed 9 color analyzer, and two Beckman Coulter XL 4-color analyzers. Despite the relocation to the Hillman Cancer Center, the usage of the flow cytometry facility has remained high, with cell sorting exclusive of setup time regularly exceeding 50% of absolute capacity (100 hours/month) and analytical cytometry exceeding 50% of business-hour capacity (160 hours/instrument/month). We anticipate that despite the loss of non-UPCI users resulting from the move, the recruitment of several new UPCI investigators and our continuing emphasis on education and training will more than make up for the loss of non-UPCI investigators.

2002年9月，UPCI流式细胞仪设备搬到了希尔曼癌症中心研究馆的新址。新实验室包括一个200英尺2的用户实验室（UserLab）和一个1000英尺2的细胞分选实验室。匹兹堡大学癌症研究所的流式细胞仪设备为UPCI内的50多个用户和20个非UPCI用户提供服务。这些服务分为五类：1)培训用户操作该设施的两台用户专用分析流式细胞仪；2)为调查人员进行高速荧光活化细胞分选；3)确保设施内的仪器每天都经过适当的校准，并就实验的适当设置向用户提供建议；4)指导研究者进行实验设计和数据分析，协助进行复杂的多参数实验；(5)将所产生的所有数据存档