CORE--FLOW CYTOMETRY FACILITY

核心——流式细胞仪

• 批准号: 6989520
• 负责人: ALBERT D DONNENBERG
• 金额: $ 10.66万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-22 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6989520
• 关键词: biomedical facility; cell cycle; cell sorting; flow cytometry; neoplasm /cancer

The UPCI Flow Cytometry Facility moved to its new location at the Hillman Cancer Center Research Pavilion in September 2002. The new laboratory consists of a 200 ft 2 user laboratory (UserLab), and a 1000 ft 2 cell sorting laboratory. The Flow Cytometry Facility of the University of Pittsburgh Cancer Institute provides services to over 50 users within the UPCI and 20 non-UPCI users. These services fall into five categories: 1) Training users to operate the Facility's two user-dedicated analytical flow cytometers; 2) Performing high speed fluorescence activated cell sorting for investigators; 3) Insuring that the instruments in the facility are properly calibrated on a daily basis and advising users concerning the proper settings for their experiments; 4) Advising investigators on experimental design and data analysis and assisting in performance of complex multi-parameter experiments; and 5) Archiving all data produced by the facility. The current instrumentation at the UPCI Flow Cytometry Facility includes a Dako-Cytomation MoFLo high speed cell sorter, equipped with a CytoShield Biocontainment hood, a Dako-Cytomation CyAn 9-color high speed 9 color analyzer, and two Beckman Coulter XL 4-color analyzers. Despite the relocation to the Hillman Cancer Center, the usage of the flow cytometry facility has remained high, with cell sorting exclusive of setup time regularly exceeding 50% of absolute capacity (100 hours/month) and analytical cytometry exceeding 50% of business-hour capacity (160 hours/instrument/month). We anticipate that despite the loss of non-UPCI users resulting from the move, the recruitment of several new UPCI investigators and our continuing emphasis on education and training will more than make up for the loss of non-UPCI investigators.

UPCI流式细胞仪设施于2002年9月移至Hillman Cancer Center Research Pavilion的新位置。新实验室由200英尺2用户实验室（UserLab）和1000英尺2个细胞分类实验室组成。匹兹堡大学癌症研究所的流式细胞仪设施为UPCI和20名非UPCI用户的50多名用户提供服务。这些服务分为五个类别：1）培训用户操作该设施的两个用户专用的分析流式细胞仪； 2）针对研究者执行高速荧光激活的细胞分选； 3）确保每天对设施中的仪器进行适当的校准，并为用户提供有关其实验的适当设置的建议； 4）向研究人员提供有关实验设计和数据分析的建议，并有助于进行复杂的多参数实验的性能； 5）存档由 设施。 UPCI流式细胞仪设施的当前仪器包括配备Cytoshield Biocontainmation Hood Hood的Dako-Cytomation Moflo高速细胞分配器，Dako-Cytomation Cyantomation Cyan 9颜色高速9颜色分析仪和两个Beckman Coulter coulter coulter coulter xl XL 4色分析仪。 尽管搬迁到希尔曼癌症中心，但流式细胞仪设施的使用仍然很高，细胞分类的设置时间常规超过了绝对容量的50％（每月100小时）的50％，分析细胞仪超过了业务小时容量的50％（160小时/仪器/仪器/仪器）。我们预计，尽管这一举动造成了非UPCI使用者的损失，但招募了几位新的UPCI调查人员，我们对教育和培训的持续强调将弥补非UPCI调查人员的损失。