SYNTHESIS OF NEW CLASS OF DEOXYNUCLEOTIDE ANALOGS

新型脱氧核苷酸类似物的合成

• 批准号: 6349399
• 负责人: JONAS KORLACH
• 金额: $ 1.73万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6349399
• 关键词: bioengineering /biomedical engineering; biological products; biomedical equipment development; biomedical resource; lasers; technology /technique

Photosensitiers such as Rose bengal (RB), photofrin (PF) and benzoporphyrin monoacid ring (BPD) are used as photosensitizers in photodynamics therapy studies on cancer tumers utilizing multiphoton fluorescence microscopy (MPM). Although one-photon photophysical characterization of those photosensitizers are extensively investigated, the wavelength-dependence of two-photon excitation cross-section is not known. We have measured the absolute two-photon excitation cross-section (?(2)) of RB, PF, and BPD in the wavelength range of 810-980 nm using ~80 fs laser pulses at 82 MHz repetition rate. At each wavelength, power-dependent measurements were conducted to make sure that the detected fluorescence is due to two-photon absorption. From the wavelength-dependence of ?(2), a favorable excitation wavelength range (i.e. minimum photobleaching) for MPM imaging can be determined.

光敏剂如玫瑰红（RB）、光敏素（PF）和 苯并卟啉单酸环（BPD）用作光敏剂， 多光子光动力学治疗肿瘤的研究 荧光显微镜（MPM）。 虽然单光子量子物理学 这些光敏剂的表征被广泛地 研究，双光子激发的波长依赖性 横截面未知。 我们测量了绝对双光子 激励截面（？(2))RB、PF和BPD的波长 810-980 nm范围，使用~80 fs激光脉冲，重复频率为82 MHz 率 在每个波长下，进行功率相关测量 以确保检测到的荧光是由于双光子 吸收 从波长依赖性？(2)一个有利的 MPM的激发波长范围（即最小光漂白） 可以确定成像。