FLUORESCENCE CORRELATION SPECTROSCOPY IN CLOSED NANOSTRUCTURED FLUID CHANNELS

封闭纳米结构流体通道中的荧光相关光谱

• 批准号: 6349432
• 负责人: JONAS KORLACH
• 金额: $ 1.38万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6349432
• 关键词: Mammalia; bioengineering /biomedical engineering; biological products; biomedical resource; drug /agent; human tissue; intestines; lasers; model design /development; technology /technique

The etiologies of the two major inflammatory bowel diseases (IBD), Crohn's disease and ulcerative colitis, remain unknown, although it seems clear that both diseases can be influenced by environmental and genetic factors which contribute to initiation, amplification and prolongation of the inflammatory response. The most commonly prescribed drugs for IBD therapy are those which generate high concentrations of 5-aminosalicylic acid (5-ASA) in the colonic lumen. Despite known efficacy of these drugs for treatment of active disease and maintenance of quiescent disease, therapeutic mechanisms remain uncertain, due to uncertainty of the site of action of 5-ASA in the colonic mucosa. The Montrose group at Johns Hopkins University has developed ratiometric microscopy methods to image 5-ASA and its major metabolite (N-acetyl-5-ASA; Ac-5-ASA) simultaneously in living mouse colonic mucosa and we have extended this technique by the use of multiphoton microscopy.

两种主要的炎症性肠病（IBD）的病因， 克罗恩病和溃疡性结肠炎，仍然未知，虽然它 很明显，这两种疾病都可能受到环境和 遗传因素，有助于启动，扩增和 延长炎症反应。 最常见的 用于IBD治疗的处方药物是那些产生高剂量的药物。 5-氨基水杨酸（5-阿萨）浓度在结肠腔。 尽管已知这些药物治疗活动性疾病的疗效 和维持静止期疾病，治疗机制仍然存在 不确定，由于5-阿萨在 结肠粘膜 约翰霍普金斯大学的蒙特罗斯小组 开发了比率显微镜方法来成像5-阿萨及其主要 代谢产物N-乙酰基-5-阿萨; Ac-5-阿萨 结肠粘膜，我们通过使用 多光子显微术