MULTI PHOTON EXCITATION OF GREEN FLUORESCENT PROTEINS
MULTI PHOTON EXCITATION OF GREEN FLUORESCENT PROTEINS
批准号:
6315380
负责人:
Joseph R. LAKOWICZ
金额:
$2.83万
依托单位国家:
美国
项目类别:
财政年份:
2000
资助国家:
美国
项目状态:
已结题
起止时间:
2000-04-01 至 2001-03-31
中文摘要
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英文摘要
Green fluorescent proteins has become widely used to study gene
expression, in cells and even in whole mammalian organisms.
Two-Photon induced fluorescence of Green Fluorescence Protein (GFP)
mutant was observed and characterized. Unexpectedly we have found
that two-photon cross-section of this GFP mutant is enormously high,
several time higher than for wild type GFP. The lifetime and
anisotropy decays were measured with one and two photon excitation and
analyzed globally. The zero time anisotropy for two photon excitation
is large than for one photon excitation by the factor 10/7.
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会议论文
Photonics-based Fluorescence Imaging for Research, Diagnostics, and Pathology
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批准号:10546493
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项目类别:
-
资助金额:$38.63万
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财政年份:2022
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负责人:Joseph R. LAKOWICZ
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依托单位:
Photonics-based Fluorescence Imaging for Research, Diagnostics, and Pathology
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批准号:10329143
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项目类别:
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资助金额:$38.63万
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财政年份:2022
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负责人:Joseph R. LAKOWICZ
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依托单位:
Coupled Emission Microscopy for the Biosciences
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批准号:9424262
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项目类别:
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资助金额:$36.28万
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财政年份:2018
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负责人:Joseph R. LAKOWICZ
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依托单位:
Plasmon-coupled Fluorescence Correlation Spectroscopy in Nanoholes
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批准号:9766321
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项目类别:
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资助金额:$19.31万
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财政年份:2018
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负责人:Joseph R. LAKOWICZ
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依托单位:
Coupled Emission Microscopy for the Biosciences
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批准号:10093077
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项目类别:
-
资助金额:$36.28万
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财政年份:2018
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负责人:Joseph R. LAKOWICZ
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依托单位:
Bioaffinity Assays Using UV One-Dimensional Photonic Crystals (1DPC)
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批准号:9098709
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项目类别:
-
资助金额:$19.3万
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财政年份:2015
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负责人:Joseph R. LAKOWICZ
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依托单位:
Multi-User Time-Resolved Fluorescence Spectrometer
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批准号:8825781
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项目类别:
-
资助金额:$24.34万
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财政年份:2015
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负责人:Joseph R. LAKOWICZ
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依托单位:
Bioaffinity Assays Using UV One-Dimensional Photonic Crystals (1DPC)
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批准号:8957305
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项目类别:
-
资助金额:$22.74万
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财政年份:2015
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负责人:Joseph R. LAKOWICZ
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依托单位:
Diffusion-Enhanced Lanthanide Nanoparticle FRET Assays
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批准号:9095386
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项目类别:
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资助金额:$19.19万
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财政年份:2014
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负责人:Joseph R. LAKOWICZ
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依托单位:
Fluorescence Lifetime Imaging Microscopy (FLIM)
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批准号:7791919
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项目类别:
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资助金额:$47.4万
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财政年份:2010
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负责人:Joseph R. LAKOWICZ
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依托单位:
Sub-Wavelength Imaging of Intracellular Metal Ions
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批准号:7940807
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项目类别:
-
资助金额:$36.12万
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财政年份:2009
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负责人:Joseph R. LAKOWICZ
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依托单位:
DNA Sequencing Using Intricsic Base Fluorescence
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批准号:8105039
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项目类别:
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资助金额:$30.0万
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财政年份:2009
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负责人:Joseph R. LAKOWICZ
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依托单位:
DNA Sequencing Using Intricsic Base Fluorescence
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批准号:7923383
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项目类别:
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资助金额:$30.0万
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财政年份:2009
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负责人:Joseph R. LAKOWICZ
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依托单位:
DNA Sequencing Using Intricsic Base Fluorescence
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批准号:7714332
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项目类别:
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资助金额:$27.97万
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财政年份:2009
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负责人:Joseph R. LAKOWICZ
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依托单位:
Sub-Wavelength Imaging of Intracellular Metal Ions
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批准号:7816175
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项目类别:
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资助金额:$49.9万
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财政年份:2009
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负责人:Joseph R. LAKOWICZ
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依托单位:
Plasmon-Controlled Fluorescence and Cardiac Markers
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批准号:7476385
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项目类别:
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资助金额:$39.2万
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财政年份:2007
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负责人:Joseph R. LAKOWICZ
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依托单位:
Plasmon-Controlled Fluorescence and Cardiac Markers
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批准号:7812211
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项目类别:
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资助金额:$21.79万
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财政年份:2007
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负责人:Joseph R. LAKOWICZ
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依托单位:
Plasmon-Controlled Fluorescence and Cardiac Markers
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批准号:7259564
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项目类别:
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资助金额:$40.0万
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财政年份:2007
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负责人:Joseph R. LAKOWICZ
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依托单位:
Plasmon-Controlled Fluorescence and Cardiac Markers
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批准号:8368147
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项目类别:
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资助金额:$34.54万
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财政年份:2007
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负责人:Joseph R. LAKOWICZ
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依托单位:
Plasmon-Controlled Fluorescence and Cardiac Markers
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批准号:7650132
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项目类别:
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资助金额:$39.2万
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财政年份:2007
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负责人:Joseph R. LAKOWICZ
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依托单位: