IN VIVO IMAGING OF NEOPLASIA

肿瘤的体内成像

• 批准号: 6135908
• 负责人: Brian D. Ross
• 金额: $ 40万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-12 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6135908
• 关键词: DNA damage; angiogenesis; apoptosis; bioimaging /biomedical imaging; bioluminescence; biomedical resource; clinical research; early diagnosis; gene mutation; human subject; immunity; laboratory mouse; magnetic resonance imaging; neoplasm /cancer diagnosis; neoplasm /cancer genetics; neoplasm /cancer immunology; neoplastic growth; neoplastic transformation; noninvasive diagnosis; p53 gene /protein; positron emission tomography

The objectives of this current planning grant are to establish the ICMIC organizational and operational infrastructure and to develop new multidisciplinary research projects. The organizational structure will consist of investigators with different technical and research interests and expertise in order to foster a significant cross-fertilization of ideas and concepts. This "brainstorming" approach has already yielded the identification of very promising and novel imaging applications. The overall approach we are taking will be to develop and test new molecular constructs that will activate reporter genes detectable by either PET or bioluminescence imaging to "report" the occurrence of specific cellular or molecular events. These events include apoptosis, p53 mutation, DNA damage, immune response, angiogenesis and therapeutic intervention. Both the initial biological event and the subsequent measured biological response (e.g. tumor cellularity/cell kill, tumor perfusion, biochemical alterations, etc.) will be noninvasively monitored in vivo. This approach will provide for a more complete understanding of the events involved in the transformation process leading to tumor initiation, progression, angiogenesis, metastasis, immune response, and response to therapy. The development of additional molecular reporter constructs is anticipated and will be made available to both the internal and external research community in an effort to foster productive interdisciplinary and unique collaborations in cancer research based upon new molecular imaging capabilities. These exciting capabilities should serve to foster new ideas in both basic biology and in therapeutic intervention. A Small Animal Tumor Imaging Resource has been developed and was recently funded by the NIH/NCI as part of the University of Michigan Comprehensive Cancer Center. This Center houses 2T and 7T horizontal bore MRI/S systems, an in vivo bioluminescent imaging system, a microCT scanner and autoradiograpkic and digital image processing cores. We propose to use this resource as a foundation for establishing an In Vivo Cellular and Molecular Imaging Center (ICMIC) at the University of Michigan. The institution has committed additional resources to assist with the establishment of the ICMIC in the form of additional imaging capabilities including a new 9.4T MRI system with microimaging capabilities and a MicroPET system. A vital aspect of planning this ICMIC is that the University of Michigan will have a unique advantage of having all of these multimodality imaging capabilities contained within a single infrastructure which will allow greater accessibility and cross-modality comparisons for cancer researchers independent of departmental affiliation.

当前计划赠款的目标是建立ICMIC组织和运营基础设施，并开发新的多学科研究项目。组织结构将包括具有不同技术和研究兴趣和专业知识的研究人员，以促进思想和概念的大量交叉侵入。这种“头脑风暴”方法已经得出了非常有前途和新颖的成像应用的识别。我们采用的总体方法是开发和测试新的分子构建体，这些构建体将激活通过PET或生物发光成像可检测到的报告基因，以“报告”特定的细胞或分子事件的发生。这些事件包括凋亡，p53突变，DNA损伤，免疫反应，血管生成和治疗干预。最初的生物学事件和随后的生物学反应（例如肿瘤细胞/细胞杀死，肿瘤灌注，生化改变等）都将在体内无创监测。这种方法将使人们对转化过程所涉及的事件有更完整的了解，从而导致肿瘤起步，进展，血管生成，转移，免疫反应以及对治疗的反应。预计将开发其他分子记者结构，并将为内部和外部研究社区提供，以促进基于新的分子成像能力的癌症研究中生产性跨学科和独特的合作。这些令人兴奋的功能应该有助于在基本生物学和治疗干预中培养新思想。已经开发了一种小型动物肿瘤成像资源，最近由NIH/NCI资助，作为密歇根大学综合癌症中心的一部分。该中心设有2T和7T水平孔MRI/S系统，一个体内生物发光成像系统，微型扫描仪和自adiograpkic和数字图像处理核心。我们建议将此资源用作建立密歇根大学的体内细胞和分子成像中心（ICMIC）的基础。该机构已投入更多资源，以其他成像功能的形式协助建立ICMIC，包括具有微型成像功能的新9.4T MRI系统和Mi​​croPET系统。计划这种ICMIC的一个至关重要的方面是，密歇根大学将具有独特的优势，即在单个基础设施中拥有所有这些多模式成像功能，该功能将允许对独立于部门隶属关系的癌症研究人员进行更大的可访问性和交叉模式比较。