Assessing neurovascular coupling with functional mapping

通过功能映射评估神经血管耦合

• 批准号: 6585227
• 负责人: Sameer Anil Sheth
• 金额: $ 2.64万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-30 至 2004-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6585227
• 关键词: bioimaging /biomedical imaging; biological signal transduction; brain circulation; brain electrical activity; brain imaging /visualization /scanning; brain mapping; electrophysiology; fiber optics; functional magnetic resonance imaging; hemodynamics; hemoglobin; laboratory rat; neurophysiology; oxygen transport; oxyhemoglobin; predoctoral investigator; respiratory oxygenation; spectrometry

DESCRIPTION (provided by applicant): The objective of this proposal is to investigate the coupling between brain activation and cerebral perfusion. The details behind this neurovascular coupling are yet unclear, but form the basis for several widely used imaging modalities such as functional magnetic resonance imaging (fMRI), positron emission tomography (PET), and optical imaging of intrinsic signals (OIS). To help clarify the properties of this relationship, this proposal outlines two specific goals: (l) to characterize the spatial and temporal evolution of hemoglobin oxygenation changes within and between vascular and parenchymal compartments; and (2) to determine how well these perfusion signals are coupled to underlying neuronal activity. OIS is well-suited for the proposed studies because if offers high spatial and temporal resolution, as well as the opportunity for simultaneous electrophysiological recording. A technique for extracting hemoglobin (Hb) concentration changes from the OIS images in two spatial dimensions is presented and, in combination with field potential recording, used to study spatial and temporal aspects of neurovascular coupling in rodent somatosensory cortex. The results of these experiments will influence the design and interpretation of perfusion-based brain imaging techniques, especially fMRI. Identifying aspects of the fMRI signal that more closely reflect underlying neuronal activity will improve the technique's ability to localize brain activity. This development would significantly increase its utility for pre-operative surgical planning, for example. Determining whether coupling breaks down in certain instances will also identify possible limitations to these techniques.

描述（由申请人提供）： 本研究的目的是探讨脑激活和脑灌注之间的耦合。这种神经血管耦合背后的细节尚不清楚，但形成了几种广泛使用的成像方式的基础，如功能性磁共振成像（fMRI），正电子发射断层扫描（PET）和内在信号的光学成像（OIS）。为了帮助阐明这种关系的性质，该提议概述了两个具体目标：（1）表征血管和实质隔室内和之间血红蛋白氧合变化的空间和时间演变;以及（2）确定这些灌注信号与潜在神经元活动的耦合程度。 OIS非常适合拟议的研究，因为它提供了高的空间和时间分辨率，以及同时进行电生理记录的机会。从OIS图像中提取血红蛋白（Hb）浓度变化的技术，在两个空间维度，结合场电位记录，用于研究啮齿动物体感皮层神经血管耦合的空间和时间方面。这些实验的结果将影响基于灌注的脑成像技术，特别是功能磁共振成像的设计和解释。识别功能性磁共振成像信号中更能反映潜在神经元活动的方面，将提高该技术定位大脑活动的能力。例如，这一发展将显著增加其在术前手术规划中的实用性。确定耦合是否在某些情况下会发生故障也将确定这些技术可能存在的局限性。