GENE SPECIFIC MUTATIONS IN SKIN INDUCED BY PEROXYNITRITE

过氧亚硝酸盐引起的皮肤基因特异性突变

• 批准号: 6376215
• 负责人: FREDIKA A ROBERTSON
• 金额: $ 29.67万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-06-01 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6376215
• 关键词: DNA damage; chemical carcinogen; chemical carcinogenesis; enzyme activity; enzyme induction /repression; gene mutation; human genetic material tag; human tissue; hydrogen peroxide; hypoxanthine phosphoribosyltransferase; inflammation; keratinocyte; nitric oxide; nitric oxide synthase; nitrites; peroxidation; peroxides; polymerase chain reaction; single strand conformation polymorphism; skin neoplasms; superoxides; tissue /cell culture; tumor suppressor genes

DESCRIPTION: The focus of this RO1 is on the induction, repair and mutagenicity of O- and N- oxidant generated DNA damage in cultured human epidermal keratinocytes. The hypothesis driving this research is that NO and peroxynitrite are mutagenic, and could be important contributors to oxidative DNA damage and mutation in human epidermal keratinocytes at sites of inflammation.

描述：本RO 1的重点是诱导、修复和 O-和N-氧化剂对培养人DNA损伤致突变性 表皮角质形成细胞 推动这项研究的假设是， 和过氧亚硝酸盐具有致突变性，可能是 人表皮角质形成细胞DNA氧化损伤和突变的研究 炎症。

项目成果

Protection of human keratinocyte mtDNA by low-level nitric oxide.

低水平一氧化氮对人角质形成细胞 mtDNA 的保护。

• DOI: 10.1006/niox.2001.0381
• 发表时间: 2001
• 期刊: Nitric oxide : biology and chemistry
• 作者: Shokolenko,I;Oberyszyn,TM;D'Ambrosio,SM;Saavedra,JE;Keefer,LK;LeDoux,SP;Wilson,GL;Robertson,FM
• 通讯作者: Robertson,FM