REGULATED ENDOCYTIC TRAFFIC IN FIBROBLASTS
成纤维细胞内吞交通的调节
基本信息
- 批准号:6283656
- 负责人:
- 金额:$ 28.82万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2001
- 资助国家:美国
- 起止时间:2001-02-15 至 2005-01-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
DESCRIPTION (Applicant's abstract): The long-term objective of this project is
to characterize the mechanism that mediates regulated, dynamic retention in
endosomes. To accomplish this goal, we will characterize in Chinese hamster
ovary (CHO) cells the endocytic behavior of vpTR, a chimera between the human
transferrin receptor and IRAP (insulinregulated aminopeptidase). We have
previously demonstrated that vpTR is trafficked by a specialized,
insulin-regulated, dynamic retention mechanism in CHO cells. The specific aims
of this proposal are to characterize the motifs that determine dynamic
retention within the endosomal system, to identify the proteins that interact
with specialized targeting motifs, and to develop a model for the molecular
mechanism of dynamic retention within the endosomes. To accomplish these
objectives we will combine site-directed mutagenesis and NMR structure analysis
to develop structure-function models for the motifs. These models will be
tested by characterizing the trafficking of mutants expressed in CHO cells. The
proteins that bind these motifs will be identified using various yeast-two
hybrid methods for detecting protein-protein interactions. A variety of intact
and semi-intact cell assays will be used to investigate the molecular mechanism
of dynamic retention. Specialized and regulated trafficking processes are key
for cell function and normal whole body physiology. Not surprisingly, it is
becoming increasingly clear that perturbations in membrane trafficking are a
common cause of disease in humans, and it is therefore important to gain an
understanding of these processes at a molecular level. The importance of
dynamic retention along the biosynthetic pathway is well appreciated, and it is
likely that dynamic retention within endosomes is equally important. Analysis
of vpTR trafficking in CHO cells provides us the opportunity to studying this
mechanism in molecular detail. The insulin-regulated, dynamic retention
mechanism in CHO cells is likely to be a more common mechanism than the
specialized insulin-regulated trafficking in fat cells. Consequently the
results of the studies in this proposal may have signficant impact on our
understanding of membrane trafficking mechanisms in general as well as being
instructive for future studies in fat and muscle cells.
描述(申请人摘要):该项目的长期目标是
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('TIMOTHY E MCGRAW', 18)}}的其他基金
Insulin control of GLUT4 traffic to the plasma membrane of adipocytes
胰岛素控制 GLUT4 向脂肪细胞质膜的运输
- 批准号:
10033361 - 财政年份:2020
- 资助金额:
$ 28.82万 - 项目类别:
Insulin control of GLUT4 traffic to the plasma membrane of adipocytes
胰岛素控制 GLUT4 向脂肪细胞质膜的运输
- 批准号:
10224692 - 财政年份:2020
- 资助金额:
$ 28.82万 - 项目类别:
Insulin control of GLUT4 traffic to the plasma membrane of adipocytes
胰岛素控制 GLUT4 向脂肪细胞质膜的运输
- 批准号:
10438682 - 财政年份:2020
- 资助金额:
$ 28.82万 - 项目类别:
Insulin control of GLUT4 traffic to the plasma membrane of adipocytes
胰岛素控制 GLUT4 向脂肪细胞质膜的运输
- 批准号:
10655330 - 财政年份:2020
- 资助金额:
$ 28.82万 - 项目类别:
GIP receptor: The role of post-activation receptor behavior for the incretin effect
GIP 受体:激活后受体行为对肠促胰岛素作用的作用
- 批准号:
9976501 - 财政年份:2018
- 资助金额:
$ 28.82万 - 项目类别:
GIP receptor: The role of post-activation receptor behavior for the incretin effect
GIP 受体:激活后受体行为对肠促胰岛素作用的作用
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10205051 - 财政年份:2018
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Functional Studies of the Incretin GIP Receptor in Adipocytes
脂肪细胞中肠促胰岛素 GIP 受体的功能研究
- 批准号:
8963463 - 财政年份:2012
- 资助金额:
$ 28.82万 - 项目类别:
Functional Studies of the Incretin GIP Receptor in Adipocytes
脂肪细胞中肠促胰岛素 GIP 受体的功能研究
- 批准号:
8585058 - 财政年份:2012
- 资助金额:
$ 28.82万 - 项目类别:
Functional Studies of the Incretin GIP Receptor in Adipocytes
脂肪细胞中肠促胰岛素 GIP 受体的功能研究
- 批准号:
8451694 - 财政年份:2012
- 资助金额:
$ 28.82万 - 项目类别:
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