Cis-regulatory Requirements for Repression by UBX

描述（由申请人提供） Hox基因是进化上古老的转录因子家族 对于所有动物的正常胚胎发育和图案形成至关重要， 包括人类尽管Hox基因很重要，但我们对Hox基因的理解， 这些基因协调大量靶细胞表达的方式 基因，从而控制发展，是不清楚的。特别是我们不知道 Hox基因调控的顺式调控要求。我们不知道什么 Hox靶向增强子需要Hox结合位点的种类是Hox响应性的， 我们也不知道这些结合位点的拓扑结构必须相对于 其他调节输入到增强子。该提案旨在利用 合成修饰的增强子，以确定数字和拓扑结构 Hox基因对靶基因调控的顺式调控要求 超双胸通过这样做，它应该增加我们对 重要的Hox基因家族控制着胚胎发育 发展，并可能阐明转录的一般原则， 调控，大型基因调控网络的控制，以及 基因调控网络的变化。

DESCRIPTION (provided by applicant) The Hox genes are an evolutionarily ancient family of transcription factors critical for proper embryonic development and pattern formation in all animals, including humans. Despite the importance of Hox genes our understanding of the way in which these genes coordinate the expression of large batteries of target genes and thereby control development, is unclear. In particular we do not know the cis-regulatory requirements for Hox gene regulation. We do not know what kind of Hox binding sites a Hox target enhancer requires to be Hox responsive, nor do we know what the topology of these binding sites must be relative to the other regulatory inputs to the enhancer. This proposal is designed to use synthetically modified enhancers to determine the numerical and topological cis-regulatory requirements for target gene regulation by the Hox gene Ultrabithorax. In so doing it should increase our understanding of the way in which the fundamentally important Hox gene family controls embryonic development and may shed light on the general principles of transcriptional regulation, the control of large genetic regulatory networks, and the evolution of genetic regulatory networks over time.