Cis-regulatory Requirements for Repression by UBX

描述(由申请人提供) HOX基因是一个进化上古老的转录因子家族 对所有动物的正常胚胎发育和图案形成至关重要， 包括人类。尽管HOX基因很重要，但我们对 这些基因协调大量靶基因表达的方式 基因，从而控制发育，目前还不清楚。特别是，我们不知道 HOX基因调控的顺式调控要求。我们不知道是什么 HOX靶向增强子需要具有HOX反应的HOX结合位点的种类， 我们也不知道这些结合位点的拓扑结构一定是相对于 对增强剂的其他监管输入。本提案旨在使用 合成修饰的增强剂以确定数值和拓扑 HOX基因对靶基因调控的顺式调控要求 超大胸腔。在这样做的过程中，应该增加我们对 其中最重要的HOX基因家族控制着胚胎 发展，并可能阐明转录的一般原则 调控、大型基因调控网络的控制和进化 基因调控网络随着时间的推移。

DESCRIPTION (provided by applicant) The Hox genes are an evolutionarily ancient family of transcription factors critical for proper embryonic development and pattern formation in all animals, including humans. Despite the importance of Hox genes our understanding of the way in which these genes coordinate the expression of large batteries of target genes and thereby control development, is unclear. In particular we do not know the cis-regulatory requirements for Hox gene regulation. We do not know what kind of Hox binding sites a Hox target enhancer requires to be Hox responsive, nor do we know what the topology of these binding sites must be relative to the other regulatory inputs to the enhancer. This proposal is designed to use synthetically modified enhancers to determine the numerical and topological cis-regulatory requirements for target gene regulation by the Hox gene Ultrabithorax. In so doing it should increase our understanding of the way in which the fundamentally important Hox gene family controls embryonic development and may shed light on the general principles of transcriptional regulation, the control of large genetic regulatory networks, and the evolution of genetic regulatory networks over time.