Nanoscale Devices on DNA Functionalized Semiconductors

DNA 功能化半导体纳米级器件

• 批准号: 6445349
• 负责人: DAVID S GINGER
• 金额: $ 3.33万
• 依托单位: NORTHWESTERN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6445349
• 关键词: DNA; atomic force microscopy; biosensor device; electron transport; fluorescence resonance energy transfer; microarray technology; nanotechnology; nucleic acid structure; oligonucleotides; printing; technology /technique development

DESCRIPTION (provided by applicant): The goal of this research program is to interface semiconductor circuitry with nanoparticle-based DNA detection schemes using the technique of Dip Pen Nanolithography (DPN). The project will develop optimize the chemistry needed to immobilize oligonucleotides on semiconductor surfaces in a manner compatible with the DPN process. Modifications of the atomic force microscope/DPN tip to enhance the efficiency of oligonucleotide ink deposition will be pursued and the DPN fabricated substrates will be used to assemble and characterize patterns of DNA functionalized nanoparticles. The project will develop and study new single-label detection schemes based on the electrical and near-field-optical properties of nanoparticles with surface-bound DNA, and will develop proof-of-principle demonstrations of integrated nanoscale biosensor circuitry. Not only do these schemes offer the promise of enhanced sensitivity and selectivity as compared to fluorophore-based detection methods, but their development also addresses fundamental issues of nanoscience in biological systems including those of diffusion, charge transport and energy transfer.

描述（由申请人提供）：该研究计划的目的是 用纳米颗粒的DNA检测方案的界面半导体电路 使用浸入纳米光刻技术（DPN）的技术。该项目将开发 优化将寡核苷酸固定在半导体上所需的化学反应 以与DPN过程兼容的方式表面。修改 原子力显微镜/DPN尖端以提高寡核苷酸的效率 将追求墨水沉积，并使用DPN制造的底物 组装和表征DNA官能化纳米颗粒的模式。这 项目将基于 纳米颗粒的电气和近场特性 表面结合的DNA，并将发展原则证明 集成的纳米级生物传感器电路。这些计划不仅提供 与 基于荧光团的检测方法，但它们的发展也解决了 生物系统中纳米科学的基本问题，包括 扩散，电荷传输和能量传递。