CAMP-DEPENDENT PROTEIN KINASE A IN NEURAL TUBE FORMATION

神经管形成中的 CAMP 依赖性蛋白激酶 A

• 批准号: 6343127
• 负责人: YONGZHAO HUANG
• 金额: $ 4.09万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-01-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6343127
• 关键词: developmental neurobiology; enzyme deficiency; gene targeting; genetically modified animals; in situ hybridization; laboratory mouse; mammalian embryology; neural plate /tube; protein kinase A; spina bifida

DESCRIPTION (Applicant's abstract reproduced verbatim): The aim of this research application is to study functional consequences of a decreased PKA activity in early mouse development. It involves analyzing a PKA-deficient mouse generated with only one functional catalytic allele left in the PKA system. This PKA-deficient mouse develops severe neural tube defects leading to spina bifida and this suggests a specific requirement for PKA in neural tube formation. The proposed project includes examination of gene expression, cell proliferation and cell death in the defective neural tube. Rescue experiments will be performed to further determine the molecular basis for the neural tube defects in the PKA-deficient mice. This research will increase our understanding of the function of PKA in embryonic development, and the molecular and cellular basis of neurulation, which may lead to improved treatment for human neural tube defects.

描述（申请人的摘要逐字复制）：本申请的目的是 研究应用是研究PKA减少的功能后果， 在小鼠早期发育中的作用。它涉及分析PKA缺陷的 PKA中只剩下一个功能性催化等位基因的小鼠 系统这种PKA缺陷小鼠发展为严重的神经管缺陷， 这表明PKA在神经管中的特异性需求 阵拟议的项目包括检查基因表达，细胞 在有缺陷的神经管中的增殖和细胞死亡。挽救实验 将进一步确定神经管的分子基础 PKA缺陷小鼠中的缺陷。这项研究将提高我们的 了解PKA在胚胎发育中的作用， 神经形成的分子和细胞基础，这可能导致改善 治疗人类神经管缺陷。