CAMP-DEPENDENT PROTEIN KINASE A IN NEURAL TUBE FORMATION

描述(申请人的摘要逐字复制)：目的 研究应用是研究PKA减少的功能后果 小鼠早期发育中的活动。它包括分析一个缺乏PKA的人 在PKA中只剩下一个功能催化等位基因的小鼠 系统。这只PKA缺陷的小鼠出现了严重的神经管缺陷，导致 脊柱裂，这表明对神经管中的PKA有特殊的要求 队形。拟议的项目包括基因表达、细胞 有缺陷的神经管中的增殖和细胞死亡。救援实验 以进一步确定神经管的分子基础 PKA缺陷小鼠的缺陷。这项研究将增加我们的 了解PKA在胚胎发育中的作用，以及 神经形成的分子和细胞基础，这可能导致改善 人类神经管缺陷的治疗。

DESCRIPTION (Applicant's abstract reproduced verbatim): The aim of this research application is to study functional consequences of a decreased PKA activity in early mouse development. It involves analyzing a PKA-deficient mouse generated with only one functional catalytic allele left in the PKA system. This PKA-deficient mouse develops severe neural tube defects leading to spina bifida and this suggests a specific requirement for PKA in neural tube formation. The proposed project includes examination of gene expression, cell proliferation and cell death in the defective neural tube. Rescue experiments will be performed to further determine the molecular basis for the neural tube defects in the PKA-deficient mice. This research will increase our understanding of the function of PKA in embryonic development, and the molecular and cellular basis of neurulation, which may lead to improved treatment for human neural tube defects.