Antioxidants and oxidative damage in mitochondria

描述（由申请人提供） 这个项目的重点在于小的抗氧化分子的作用 线粒体中硝化蛋白的预防和/或修复。 蛋白 硝化作用发生在暴露于一氧化氮的生物系统中。 添加 硝基基团的酪氨酸残基，产生3-硝基酪氨酸，已被 声称是一种翻译后修饰，信号转导的一部分， 途径，或“正常”氧化应激的结果。 本研究 研究人员将描述硝化的基本机制， 蛋白质在产生一氧化氮的线粒体，鉴于重要的作用， 这些细胞器具有维持细胞ATP、β-氧化、血红素 合成和其他功能。 这些研究旨在了解 硝化作用引起的动力学和结构变化，以及如何自然地- 水果和蔬菜中存在的抗氧化剂可以防止/逆转 这种效果。 类黄酮、香豆素和儿茶素的抗氧化作用 将在羟基取代，环的共轭和O- 羟基的甲基化，以阐明结构之间的关联 和功能

DESCRIPTION (provided by applicant) The focus of this project resides on the role of small antioxidant molecules on the prevention and/or repair of nitrated proteins in mitochondria. Protein nitration occurs in biological systems exposed to nitric oxide. The addition of a nitro group to a tyrosine residue, yielding 3-nitrotyorosine, has been claimed to be a post-translational modification, part of signal transduction pathways, or a result of "normal" oxidative stress. In this study, the investigators will characterize the mechanism underlying the nitration of proteins in nitric oxide-producing mitochondria, given the important role that these organelles have in the maintenance of cellular ATP, beta-oxidation, heme synthesis, and other functions. The studies will be aimed at understanding the kinetic and structural changes caused by nitration, and how naturally- occurring antioxidants present in fruits and vegetables may prevent/reverse this effect. The antioxidant effect of flavonoids, coumarins, and catechins will be studied in terms hydroxyl substitution, conjugation of rings, and o- methylation of hydroxyl groups to elucidate an association between structure and function.