Antioxidants and oxidative damage in mitochondria

线粒体中的抗氧化剂和氧化损伤

• 批准号: 6524832
• 负责人: CECILIA GIULIVI
• 金额: $ 7.31万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-30 至 2004-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6524832
• 关键词: alpha benzopyrone; antioxidants; chemical conjugate; chemical structure function; chemical substitution; flavonoids; laboratory rat; methylation; mitochondria; nitric oxide; oxidative stress

DESCRIPTION (provided by applicant) The focus of this project resides on the role of small antioxidant molecules on the prevention and/or repair of nitrated proteins in mitochondria. Protein nitration occurs in biological systems exposed to nitric oxide. The addition of a nitro group to a tyrosine residue, yielding 3-nitrotyorosine, has been claimed to be a post-translational modification, part of signal transduction pathways, or a result of "normal" oxidative stress. In this study, the investigators will characterize the mechanism underlying the nitration of proteins in nitric oxide-producing mitochondria, given the important role that these organelles have in the maintenance of cellular ATP, beta-oxidation, heme synthesis, and other functions. The studies will be aimed at understanding the kinetic and structural changes caused by nitration, and how naturally- occurring antioxidants present in fruits and vegetables may prevent/reverse this effect. The antioxidant effect of flavonoids, coumarins, and catechins will be studied in terms hydroxyl substitution, conjugation of rings, and o- methylation of hydroxyl groups to elucidate an association between structure and function.

描述（由申请人提供）