Electron Paramagnetic Resonance Spectrometer

描述（由申请人提供）：本提案的长期目标是建立一个专门用于生物医学的电子自旋共振（ESR）设施。ESR设施将处理自由基生物学/化学与医学之间的接口研究，这是一个充满活力的领域，它在过去10年中一直在大力加强。该基金将根据这一愿景创建，旨在解决多学科科学交叉路口出现的研究机会。为此，一个由用户和专家组成的多学科团队已经从校园的各个部门（分子生物科学，免疫学和微生物学，生物化学和分子医学，生物化学，肺和重症监护，机械和航空工程，营养）招募，代表四个不同的学院/学校（兽医，医学，农业和工程），对自由基医学感兴趣。教师将使用该仪器在以下几个领域进行研究：a）抗氧化剂（如维生素E、C和类黄酮）的基础化学和生物化学; B）疟疾寄生虫蚊子载体中NO产生的生物化学和亚硝基血红蛋白的假定抗寄生虫作用; c）氧化作用，1型兰尼碱受体中的S-亚硝化/S-谷胱甘肽化; d）一氧化氮作为细胞呼吸的重要调节剂和氧梯度再分布的介体; e）热量限制和衰老期间的活性氧和氮自由基; f）自由基氧化中的铜和锌缺乏; g）作为氧化应激标志物的自由基（例如，抗坏血酸、谷胱甘肽和生育酚自由基）; h）作为神经元和肿瘤组织中细胞凋亡引发剂的自由基; i）鉴定纳米颗粒表面的结合自由基，以及这些自由基如何影响健康; j）心肌缺血/再灌注和暂时缺氧中的自由基。这些研究将在纳米科学、材料化学和生物技术/生物医学等多个领域产生影响。该提案要求购买一台最先进的ESR光谱仪，该光谱仪能够检测小的含水样品、细胞和组织切片。通过在校园建立这样的设施，我们将提供专业知识和仪器，以追求处理氧和氮自由基，碳中心自由基，影响细胞，组织和整个生物体的整体氧化还原环境的相关氧化剂和抗氧化剂的研究问题。其中自由基已被证明起关键作用的生物学相关情况从信号转导途径扩展到细胞凋亡、衰老、糖尿病、阿尔茨海默氏症以及其他病理生理学情况。我们的主要目标是推进自由基生物学/化学机制的基本理解，以便通过预防和治疗疾病的创新方法改善人类健康。公共卫生相关性：该提案要求购买一台能够检测小型水溶液样品、细胞和组织切片的ESR光谱仪。通过在校园建立这样的设施，我们将提供专业知识和仪器，以追求与人类医学相关的研究问题。所提出的实验只能使用ESR来处理氧和氮自由基在各种医学相关情况下的表征，从衰老，缺血再灌注，饮食不足到环境暴露。

DESCRIPTION (provided by applicant): The long-term goal of this proposal is to establish an Electron Spin Resonance (ESR) facility dedicated to biomedicine. The ESR Facility will address research at the interface between free radical biology/chemistry and medicine, a dynamic area that it has been intensifying extensively in the last 10 years. The Facility will be created on this vision and is designed to address the emerging research opportunities at the crossroad of multidisciplinary sciences. To this end, a multidisciplinary team of users and experts has been recruited from various Departments across Campus (Molecular Biosciences, Immunology & Microbiology, Biochemistry & Molecular Medicine, Biological Chemistry, Pulmonary & Critical Care, Mechanical & Aeronautical Engineering, and Nutrition), representing four different Colleges/Schools (Veterinary Medicine, Medicine, Agriculture, and Engineering) with interests in free radical medicine. Faculty will use this instrument to carry out research in several areas as follows: a) fundamental chemistry and biochemistry of antioxidants such as vitamin E, C and flavonoids; b) biochemistry of NO production in mosquito vectors of malaria parasites and the putative antiparasite role of nitrosylhemoglobin; c) role of the oxidation, S-nitrosation/S-glutathionylation in the ryanodine receptor type 1; d) nitric oxide as an important modulator of cell respiration and mediator of oxygen gradient redistribution; e) reactive oxygen and nitrogen radicals during caloric restriction and aging; f) copper and zinc deficiencies in free radical oxidations; g) radicals as markers of oxidative stress (e.g, ascorbyl, glutathionyl, and tocopheryl radicals); h) free radicals as initiators of apoptosis in neurons and neoplastic tissues; i) identification of bound radicals at the surface of nanoparticles, and how these affect health; j) free radicals in myocardial ischemia/reperfusion and temporary hypoxia. These studies will have an impact in a number of areas, from nanoscience to materials chemistry and biotechnology/biomedicine. This proposal requests the purchase of a state-of-the-art ESR spectrometer with capabilities for small, aqueous samples, cells, and tissue slices. By establishing such a Facility on Campus, we will provide the expertise and instrumentation to pursue research questions dealing with oxygen- and nitrogen-free radicals, carbon-centered radicals, related oxidants and antioxidants that influence the overall redox milieu of cells, tissues, and whole organisms. Biological relevant situations in which free radicals had been shown to play a critical role expand from signal transduction pathways to apoptosis, aging, diabetes, Alzheimer's, among other pathophysiological situations. Our primary goal is to advance the fundamental understanding of free radical biology/chemistry mechanisms so that human health can be improved through innovative approaches in the prevention and treatment of disease. PUBLIC HEALTH RELEVANCE: This proposal requests the purchase of an ESR spectrometer with capabilities for small, aqueous samples, cells, and tissue slices. By establishing such a Facility on Campus, we will provide the expertise and instrumentation to pursue research questions relevant to human medicine. The proposed experiments that only could be done using an ESR deal with the characterization of oxygen- and nitrogen-free radicals in a variety of medically relevant situations from aging, ischemia-reperfusion, and dietary deficiencies to environmental exposures.