Electron Paramagnetic Resonance Spectrometer

描述(由申请人提供)：这项提案的长期目标是建立一个专门用于生物医学的电子自旋共振(ESR)设施。电子自旋共振基金将致力于自由基生物/化学和医学之间的界面研究，这是一个在过去10年里广泛加强的动态领域。该基金将根据这一愿景创建，旨在处理多学科科学交叉路口出现的研究机会。为此，从校园的不同部门(分子生物科学、免疫学和微生物学、生物化学和分子医学、生物化学、肺部和危重护理、机械和航空工程以及营养学)招募了一个由用户和专家组成的多学科团队，代表四个不同的学院/学校(兽医、医学、农业和工程学)对自由基医学感兴趣。教职员工将利用该仪器开展下列领域的研究：a)抗氧化剂的基础化学和生物化学，如维生素E、C和类黄酮类；b)在疟疾寄生虫蚊媒中产生NO的生物化学以及亚硝酸血红蛋白可能的抗寄生虫作用；c)氧化作用，S亚硝化/S谷胱甘肽基化在兰尼定受体1型中的作用；d)一氧化氮作为细胞呼吸的重要调节器和氧梯度重新分配的媒介；e)热量限制和衰老过程中的活性氧和氮自由基；f)铜和锌在自由基氧化中的缺乏；G)作为氧化应激标志的自由基(如抗坏血酸、谷胱甘肽和生育基自由基)；h)作为神经元和肿瘤组织中细胞凋亡启动者的自由基；i)在纳米颗粒表面识别结合的自由基，以及这些自由基如何影响健康；j)心肌缺血/再灌流和暂时缺氧中的自由基。这些研究将在许多领域产生影响，从纳米科学到材料化学和生物技术/生物医学。这项提案要求购买一台最先进的ESR光谱仪，具有测量小样本、水样、细胞和组织切片的能力。通过在校园建立这样的设施，我们将提供专业知识和仪器来研究影响细胞、组织和整个生物体整体氧化还原环境的氧和氮自由基、以碳为中心的自由基、相关氧化剂和抗氧化剂。已被证明发挥关键作用的生物相关情况从信号转导途径扩展到细胞凋亡、衰老、糖尿病、阿尔茨海默氏症等病理生理情况。我们的主要目标是促进对自由基生物学/化学机制的基本了解，以便通过在疾病预防和治疗方面的创新方法来改善人类健康。与公共健康相关：该提案要求购买一台ESR光谱仪，该光谱仪具有测量小样本、水样、细胞和组织切片的能力。通过在校园建立这样一个设施，我们将提供专业知识和仪器来研究与人类医学相关的问题。拟议中的实验只能使用ESR来完成，涉及到从衰老、缺血再灌注、饮食缺乏到环境暴露等各种医学相关情况下氧和氮自由基的特征。

DESCRIPTION (provided by applicant): The long-term goal of this proposal is to establish an Electron Spin Resonance (ESR) facility dedicated to biomedicine. The ESR Facility will address research at the interface between free radical biology/chemistry and medicine, a dynamic area that it has been intensifying extensively in the last 10 years. The Facility will be created on this vision and is designed to address the emerging research opportunities at the crossroad of multidisciplinary sciences. To this end, a multidisciplinary team of users and experts has been recruited from various Departments across Campus (Molecular Biosciences, Immunology & Microbiology, Biochemistry & Molecular Medicine, Biological Chemistry, Pulmonary & Critical Care, Mechanical & Aeronautical Engineering, and Nutrition), representing four different Colleges/Schools (Veterinary Medicine, Medicine, Agriculture, and Engineering) with interests in free radical medicine. Faculty will use this instrument to carry out research in several areas as follows: a) fundamental chemistry and biochemistry of antioxidants such as vitamin E, C and flavonoids; b) biochemistry of NO production in mosquito vectors of malaria parasites and the putative antiparasite role of nitrosylhemoglobin; c) role of the oxidation, S-nitrosation/S-glutathionylation in the ryanodine receptor type 1; d) nitric oxide as an important modulator of cell respiration and mediator of oxygen gradient redistribution; e) reactive oxygen and nitrogen radicals during caloric restriction and aging; f) copper and zinc deficiencies in free radical oxidations; g) radicals as markers of oxidative stress (e.g, ascorbyl, glutathionyl, and tocopheryl radicals); h) free radicals as initiators of apoptosis in neurons and neoplastic tissues; i) identification of bound radicals at the surface of nanoparticles, and how these affect health; j) free radicals in myocardial ischemia/reperfusion and temporary hypoxia. These studies will have an impact in a number of areas, from nanoscience to materials chemistry and biotechnology/biomedicine. This proposal requests the purchase of a state-of-the-art ESR spectrometer with capabilities for small, aqueous samples, cells, and tissue slices. By establishing such a Facility on Campus, we will provide the expertise and instrumentation to pursue research questions dealing with oxygen- and nitrogen-free radicals, carbon-centered radicals, related oxidants and antioxidants that influence the overall redox milieu of cells, tissues, and whole organisms. Biological relevant situations in which free radicals had been shown to play a critical role expand from signal transduction pathways to apoptosis, aging, diabetes, Alzheimer's, among other pathophysiological situations. Our primary goal is to advance the fundamental understanding of free radical biology/chemistry mechanisms so that human health can be improved through innovative approaches in the prevention and treatment of disease. PUBLIC HEALTH RELEVANCE: This proposal requests the purchase of an ESR spectrometer with capabilities for small, aqueous samples, cells, and tissue slices. By establishing such a Facility on Campus, we will provide the expertise and instrumentation to pursue research questions relevant to human medicine. The proposed experiments that only could be done using an ESR deal with the characterization of oxygen- and nitrogen-free radicals in a variety of medically relevant situations from aging, ischemia-reperfusion, and dietary deficiencies to environmental exposures.