ORAL MUCOSAL CELLS, CANDIDA AND CYTOKINE PRODUCTION

描述：本项目的具体目标包括：1)研究 原代口腔上皮细胞的促炎细胞因子分泌反应 培养对白色念珠菌的挑战，包括产生IL-6和IL-8以及 与细胞因子反应相关的寄生虫因子的测定；2) 研究宿主细胞--C。白念珠菌的相互作用导致 促炎细胞因子反应，包括直接接触的必要性和 甘露糖介导的信号在诱导IL-6和IL-8反应中的作用 IL-6和IL-8可能的二次细胞因子信号的评估 合成；3)确定中性粒细胞是否具有抗真菌活性 由白色念珠菌与口腔上皮细胞相互作用调节，包括 白念珠菌吞噬功能的激活和杀菌性中性粒细胞的增强 上皮细胞分泌促炎细胞因子的活性 白念珠菌攻击的细胞。

DESCRIPTION: The specific aims of this project include: 1) to study proinflammatory cytokine secretory responses of primary oral epithelial cell cultures to C. albicans challenge, including production of IL-6 and IL-8 and determination of parasite factors associated with cytokine response; 2) to study mechanisms of host cell-C. albicans interactions which lead to proinflammatory cytokine responses, including necessity for direct contact and mannose-mediated signaling in eliciting IL-6 and IL-8 responses, as well as assessment of possible secondary cytokine-generated signals for IL-6 and IL-8 synthesis; and 3) to determine whether neutrophil anti-fungal activity can be regulated by interaction of C. albicans with oral epithelial cells, including activation of C. albicans phagocytosis and enhancement of fungicidal neutrophil activity in response to the proinflammatory cytokines secreted by epithelial cells challenged with C. albicans.