INNATE IMMUNITY IN VERTEBRATES AND INSECTS

脊椎动物和昆虫的先天免疫

• 批准号: 6374024
• 负责人: Alan B Ezekowitz
• 金额: $ 85.24万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6374024
• 关键词: immunity; immunoregulation

The goal of this Program Project Grant is to use a combination of insect and vertebrate systems to understand the origins of the human system of defense against infection. The Principal Investigators have established collaborative interactions which have been highly productive under the auspices of the Human Frontiers in Science Program. Our success results from continual interaction and sharing of expertise that has allowed for novel insights into the field of innate immunity. This Program is an extension and refinement of our collective goal which is to have a real impact on the understanding of innate immunity. To this end, we propose three projects involving six laboratories within four institutions. Project 1 aims to define molecules that are necessary for phagocytosis. Our work on the Drosophila phagocytic receptor croquemort is presented as a platform from which we plan to use Drosophila as a model system to identify genes that are necessary for phagocytosis. The genetic screens described in Project 1 will be complemented by the genetic screens outlined in Project 3. The ultimate goal is to apply this knowledge to mammalian systems in collaboration with Project 2. The goal of Project 2 is a complete analysis of our recent discovery of a human homologue of the Drosophila Toll. This molecule appears to play a pivotal role in linking innate and adaptive immunity in mammalians. Project 3 focuses on two dipteran insects, Drosophila melangaster and Anopheles gambiae. The plan is to identify, characterize and define the model of action of pattern recognition molecules that recognize infectious agents, particularly the protozoans parasite Plasmodium malariae. We propose an Administrative Core that will coordinate communications between investigators and oversee the administration of the grant. We believe that from insects we are able to apply the power of genetic approaches and the unique ability to manipulate responses that cannot be obtained in vertebrates. We plan to use mammalian systems to place this in a context that will impact human disease. This knowledge will provide insights into the links between innate and adaptive immunity which in turn will cast light on pathogen host interactions.

该计划项目赠款的目标是结合昆虫 和脊椎动物系统，以了解人类系统的起源 防御感染。主要调查人员已经建立了 协作互动在 科学计划中人类前沿的主持人。我们的成功结果 从不断的互动和分享专业知识的共享 对先天免疫领域的新颖见解。这个程序是 扩展和改进我们的集体目标，即具有真实的目标 影响对先天免疫力的理解。为此，我们提出 三个涉及四个机构内六个实验室的项目。 项目1旨在定义吞噬作用所必需的分子。 我们在果蝇吞噬受体Croquemort上的工作被视为 我们计划使用果蝇作为模型系统的平台 鉴定吞噬作用所必需的基因。遗传筛选 项目1中描述的遗传筛选将补充 项目3中概述了。最终目标是将此知识应用于 哺乳动物系统与项目2合作。项目2的目标 是对我们最近发现的人类同源物的完整分析 果蝇收费。该分子似乎在链接中起关键作用 哺乳动物的先天和适应性免疫。项目3专注于两个 双翅目昆虫，果蝇和冈比亚果蝇。计划 是识别，表征和定义模式的作用模型 识别识别传染剂的识别分子，尤其是 原生动物寄生虫疟原虫疟疾。我们提出了一个行政核心 这将协调调查人员之间的通信，并监督 赠款的管理。我们相信，从昆虫那里我们可以 运用遗传方法的力量和操纵独特的能力 无法在脊椎动物中获得的反应。我们计划使用哺乳动物 将其放置在影响人类疾病的情况下的系统。这 知识将提供有关先天和适应性之间联系的见解 免疫力反过来将揭示病原体宿主相互作用。