Pattern Recognition Molecules in Drosophila and Humans

果蝇和人类的模式识别分子

• 批准号: 6788679
• 负责人: Alan B Ezekowitz
• 金额: $ 37.96万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6788679
• 关键词: Drosophilidae; SDS polyacrylamide gel electrophoresis; anthrax; biological signal transduction; flow cytometry; human tissue; immunity; immunocytochemistry; in situ hybridization; ligands; macrophage; peptidoglycan; polymerase chain reaction; protein binding; protein structure function; receptor; spores

The central unifying theme of this Project is to define the structure and function of pattern recognition receptors in Drosophila and man. We build on our past expertise in the Laboratory of Developmental Immunology where we have developed an approach that integrates biochemistry, cell biology, genetics, proteomics, and structural biology to identify and characterize biologically relevant pattern recognition molecules. We plan to build on and extend our previous work that has benefited from the collaboration between the four laboratories and an administrative core of the PPG. We have three aims: (1) explore the structure and function of a novel pattern recognition receptor Eater (in collaboration with Projects 2, 3 and 4); (2) utilize a combination of approaches in Drosophila and man to identify the macrophage receptor for anthrax spores; (3) examine the structure and function of peptidoglycan binding proteins in flies and man (in collaboration with Projects 2, 3 and 4)

该项目的主要统一主题是定义果蝇和人类中模式识别受体的结构和功能。我们基于发育免疫学实验室的过去专业知识，在该实验室中，我们开发了一种将生物化学，细胞生物学，遗传学，蛋白质组学和结构生物学整合的方法，以识别和表征与生物学相关的模式识别分子。我们计划在四个实验室和PPG的行政核心之间的合作中受益于以前的工作并扩展我们以前的工作。我们有三个目的：（1）探索新型模式识别受体食客的结构和功能（与项目2、3和4合作）； （2）利用果蝇和人的方法组合来鉴定炭疽孢子的巨噬细胞受体； （3）检查肽和人中肽聚糖结合蛋白的结构和功能（与项目2、3和4合作）