TRYPANOSOME FLAGELLAR CALCIUM ACYL SWITCH PROTEINS

描述（改编自申请人的摘要）：免疫原性24 kDa钙 在克氏锥虫中发现一种结合蛋白，称为鞭毛钙 结合蛋白（FCaBP）。FcaBP通过添加肉豆蔻酸酯和 棕榈酸酯到其氨基末端。这些修饰介导其定位 到鞭毛质膜上钙结合也是必需的， 本地化因此，FcaBP类似于钙-肉豆蔻酰开关中的恢复 哺乳动物视网膜的蛋白质。FcaBP通过第三和第三个结合钙， 第四个EF手域与recoverin的区别，仅由 肉豆蔻酸酯并通过第二和第三EF手结构域结合钙。FcaBP 是一种新型钙酰基转换蛋白。我们将把我们对FcaBP的研究扩展到 一项为期五年的研究工作，其具体目标是：（一）确定 的鞭毛膜定位的FcaBP的机制，（ii） FCaBP伴侣蛋白的鉴定，（iii）阐明 肉豆蔻酸钙/棕榈酰转换机制和（iv）表征 布氏典型线虫钙酰基转换蛋白。

DESCRIPTION (Adapted from Applicant's Abstract): An immunogenic 24 kDa calcium binding protein is found in Trypanosoma cruzi and is termed flagellar calcium binding protein (FCaBP). FcaBP is modified by the addition of myristate and palmitate to its amino terminus. These modifications mediate its localization to the flagellar plasma membrane. Calcium binding is also required for localization. Thus, FcaBP is similar to recoverin a calcium-myristoyl switch protein of the mammalian retina. FcaBP binds calcium through the third and fourth EF hand domains in distinction from recoverin which is modified only by myristate and binds calcium through the second and third EF hand domains. FcaBP is a novel calcium-acyl switch protein. We will extend our studies of FcaBP in a five-year research effort, the Specific Aims of which are: (i) determination of the mechanism for the flagellar membrane localization of FcaBP, (ii) identification of FCaBP partner proteins, (iii) elucidation of the calcium-myristoly/palmitoyl switch mechanism and (iv) characterization of Typanosoma brucei calcium acyl switch proteins.