TRYPANOSOME FLAGELLAR CALCIUM ACYL SWITCH PROTEINS

描述(摘自申请者摘要)：一种免疫原性24 kDa钙 结合蛋白存在于克氏锥虫体内，被称为鞭毛钙 结合蛋白(FCaBP)。FcaBP通过添加肉豆蔻酸酯和 棕榈酸至其氨基末端。这些修改调解了它的本地化 至鞭毛质膜。钙结合也是必需的 本地化。因此，FcaBP类似于钙-肉豆蔻基开关的恢复 哺乳动物视网膜的蛋白质。FcaBP通过第三层和第三层结合钙 第四个EF手性结构域不同于Recovery In，后者仅由 肉豆蔻酸并通过第二和第三EF手性结构域结合钙。FcaBP 是一种新的钙-酰基转换蛋白。我们将把对FcaBP的研究扩展到 一项为期五年的研究工作，其具体目标是：(一)决心 FcaBP鞭毛膜定位机制的研究(II) FCaBP配对蛋白的鉴定(III) 钙-肉豆蔻醇/棕榈酰基开关机制及(Iv)表征 布鲁氏锥虫钙酰基开关蛋白。

DESCRIPTION (Adapted from Applicant's Abstract): An immunogenic 24 kDa calcium binding protein is found in Trypanosoma cruzi and is termed flagellar calcium binding protein (FCaBP). FcaBP is modified by the addition of myristate and palmitate to its amino terminus. These modifications mediate its localization to the flagellar plasma membrane. Calcium binding is also required for localization. Thus, FcaBP is similar to recoverin a calcium-myristoyl switch protein of the mammalian retina. FcaBP binds calcium through the third and fourth EF hand domains in distinction from recoverin which is modified only by myristate and binds calcium through the second and third EF hand domains. FcaBP is a novel calcium-acyl switch protein. We will extend our studies of FcaBP in a five-year research effort, the Specific Aims of which are: (i) determination of the mechanism for the flagellar membrane localization of FcaBP, (ii) identification of FCaBP partner proteins, (iii) elucidation of the calcium-myristoly/palmitoyl switch mechanism and (iv) characterization of Typanosoma brucei calcium acyl switch proteins.