Transgenic engineering of Aedine mosquitoes

伊丁蚊的转基因工程

基本信息

  • 批准号:
    6332193
  • 负责人:
  • 金额:
    $ 22.1万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2001
  • 资助国家:
    美国
  • 起止时间:
    2001-05-01 至 2006-03-31
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): Mosquito-borne infectious diseases continue to have significant impact on the health and economy of much of the world. Alternative strategies for control of mosquito vectors could arise through a greater understanding of the genetics and physiology of these insects. Progress in genetic research with mosquitoes will be greatly enhanced by the development of effective and reproducible means of transgenic manipulations. A key component of this capability is the identification and manipulation of insect transposons that are able to efficiently vector genes into the mosquito genome, permit gene tagging, and maintain genes in an active state. The convergence of research in this field has resulted in the successful transgenic manipulation of mosquitoes with several insect transposons, each with its own unique properties. The insect transposon piggyBac has been demonstrated to be effective for genetic manipulation in widely diverse insect species, including mosquitoes. Recently, we demonstrated transformation of Aedes acgypti with piggyBac using the cinnabar eye coloration marker gene. This proposal seeks significant funding to understand and exploit the capabilities of the piggyBac transposon for establishing and maintaining transgenic strains of Aedine mosquito species. Additional piggyBac-based transgene vectors containing desirable genes under the control of either ubiquitous insect promoters or tissue-specific mosquito promoters will be constructed, tested, and combined with the selectable markers for transgenesis. The presence and expression of the transposon and vectored genes will be detected following selection for the co-transforming marker gene at the Gi generation, and analyzed using a variety of techniques including Southern hybridization, PCR amplification and sequencing, Northern and Western analyses, and immunoflourescence, as appropriate. Genetic characterization of the stability and movement of transgene sequences within the mosquito genome will be examined over several generations. This research will provide an exciting and much needed capability for significant enhancement of the basic research effort in mosquito genetics and physiology.
描述(申请人提供):蚊媒传染病

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(1)

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Malcolm J. FRASER其他文献

Malcolm J. FRASER的其他文献

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{{ truncateString('Malcolm J. FRASER', 18)}}的其他基金

Engineering optimized N-glycosylation in the silkworm silkgland protein expression system
家蚕丝腺蛋白表达系统中的工程优化 N-糖基化
  • 批准号:
    10380639
  • 财政年份:
    2019
  • 资助金额:
    $ 22.1万
  • 项目类别:
Engineering optimized N-glycosylation in the silkworm silkgland protein expression system
家蚕丝腺蛋白表达系统中的工程优化 N-糖基化
  • 批准号:
    9982365
  • 财政年份:
    2019
  • 资助金额:
    $ 22.1万
  • 项目类别:
Developing Anti-viral Ribozymes to Suppress Arboviruses in Transgenic Mosquitoes
开发抗病毒核酶来抑制转基因蚊子中的虫媒病毒
  • 批准号:
    8968808
  • 财政年份:
    2011
  • 资助金额:
    $ 22.1万
  • 项目类别:
Developing Anti-viral Ribozymes to Suppress Arboviruses in Transgenic Mosquitoes
开发抗病毒核酶来抑制转基因蚊子中的虫媒病毒
  • 批准号:
    8760304
  • 财政年份:
    2011
  • 资助金额:
    $ 22.1万
  • 项目类别:
Developing Anti-viral Ribozymes to Suppress Arboviruses in Transgenic Mosquitoes
开发抗病毒核酶来抑制转基因蚊子中的虫媒病毒
  • 批准号:
    8581314
  • 财政年份:
    2011
  • 资助金额:
    $ 22.1万
  • 项目类别:
Developing Anti-viral Ribozymes to Suppress Arboviruses in Transgenic Mosquitoes
开发抗病毒核酶来抑制转基因蚊子中的虫媒病毒
  • 批准号:
    8390473
  • 财政年份:
    2011
  • 资助金额:
    $ 22.1万
  • 项目类别:
Developing Anti-viral Ribozymes to Suppress Arboviruses in Transgenic Mosquitoes
开发抗病毒核酶来抑制转基因蚊子中的虫媒病毒
  • 批准号:
    8222320
  • 财政年份:
    2011
  • 资助金额:
    $ 22.1万
  • 项目类别:
Transgenic engineering of Aedine mosquitoes
伊丁蚊的转基因工程
  • 批准号:
    6880028
  • 财政年份:
    2001
  • 资助金额:
    $ 22.1万
  • 项目类别:
Transgenic engineering of Aedine mosquitoes
伊丁蚊的转基因工程
  • 批准号:
    6632427
  • 财政年份:
    2001
  • 资助金额:
    $ 22.1万
  • 项目类别:
Transgenic engineering of Aedine mosquitoes
伊丁蚊的转基因工程
  • 批准号:
    6738965
  • 财政年份:
    2001
  • 资助金额:
    $ 22.1万
  • 项目类别:
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