Transgenic engineering of Aedine mosquitoes
伊丁蚊的转基因工程
基本信息
- 批准号:6880028
- 负责人:
- 金额:$ 42.87万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2001
- 资助国家:美国
- 起止时间:2001-05-01 至 2008-03-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
DESCRIPTION (provided by applicant): Mosquito-borne infectious diseases
continue to have significant impact on the health and economy of much of the
world. Alternative strategies for control of mosquito vectors could arise
through a greater understanding of the genetics and physiology of these
insects. Progress in genetic research with mosquitoes will be greatly enhanced
by the development of effective and reproducible means of transgenic
manipulations. A key component of this capability is the identification and
manipulation of insect transposons that are able to efficiently vector genes
into the mosquito genome, permit gene tagging, and maintain genes in an active
state. The convergence of research in this field has resulted in the successful
transgenic manipulation of mosquitoes with several insect transposons, each
with its own unique properties. The insect transposon piggyBac has been
demonstrated to be effective for genetic manipulation in widely diverse insect
species, including mosquitoes. Recently, we demonstrated transformation of
Aedes acgypti with piggyBac using the cinnabar eye coloration marker gene. This
proposal seeks significant funding to understand and exploit the capabilities
of the piggyBac transposon for establishing and maintaining transgenic strains
of Aedine mosquito species. Additional piggyBac-based transgene vectors
containing desirable genes under the control of either ubiquitous insect
promoters or tissue-specific mosquito promoters will be constructed, tested,
and combined with the selectable markers for transgenesis. The presence and
expression of the transposon and vectored genes will be detected following
selection for the co-transforming marker gene at the Gi generation, and
analyzed using a variety of techniques including Southern hybridization, PCR
amplification and sequencing, Northern and Western analyses, and
immunoflourescence, as appropriate. Genetic characterization of the stability
and movement of transgene sequences within the mosquito genome will be examined
over several generations. This research will provide an exciting and much
needed capability for significant enhancement of the basic research effort in
mosquito genetics and physiology.
描述(申请人提供):蚊媒传染病
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Malcolm J. FRASER其他文献
Malcolm J. FRASER的其他文献
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{{ truncateString('Malcolm J. FRASER', 18)}}的其他基金
Engineering optimized N-glycosylation in the silkworm silkgland protein expression system
家蚕丝腺蛋白表达系统中的工程优化 N-糖基化
- 批准号:
10380639 - 财政年份:2019
- 资助金额:
$ 42.87万 - 项目类别:
Engineering optimized N-glycosylation in the silkworm silkgland protein expression system
家蚕丝腺蛋白表达系统中的工程优化 N-糖基化
- 批准号:
9982365 - 财政年份:2019
- 资助金额:
$ 42.87万 - 项目类别:
Developing Anti-viral Ribozymes to Suppress Arboviruses in Transgenic Mosquitoes
开发抗病毒核酶来抑制转基因蚊子中的虫媒病毒
- 批准号:
8760304 - 财政年份:2011
- 资助金额:
$ 42.87万 - 项目类别:
Developing Anti-viral Ribozymes to Suppress Arboviruses in Transgenic Mosquitoes
开发抗病毒核酶来抑制转基因蚊子中的虫媒病毒
- 批准号:
8968808 - 财政年份:2011
- 资助金额:
$ 42.87万 - 项目类别:
Developing Anti-viral Ribozymes to Suppress Arboviruses in Transgenic Mosquitoes
开发抗病毒核酶来抑制转基因蚊子中的虫媒病毒
- 批准号:
8581314 - 财政年份:2011
- 资助金额:
$ 42.87万 - 项目类别:
Developing Anti-viral Ribozymes to Suppress Arboviruses in Transgenic Mosquitoes
开发抗病毒核酶来抑制转基因蚊子中的虫媒病毒
- 批准号:
8390473 - 财政年份:2011
- 资助金额:
$ 42.87万 - 项目类别:
Developing Anti-viral Ribozymes to Suppress Arboviruses in Transgenic Mosquitoes
开发抗病毒核酶来抑制转基因蚊子中的虫媒病毒
- 批准号:
8222320 - 财政年份:2011
- 资助金额:
$ 42.87万 - 项目类别: