CORE--MOLECULAR AND CELLULAR ASSAY FACILITY

产品说明：（申请人描述）核心A，分子和细胞测定的长期目标是[1]提供WRN野生型、杂合和无效人和小鼠源性细胞、细胞系或本项目产生的组织的基因型和表型表征，[2]为项目项目采购和储存肉瘤和脑膜瘤，以便进行WRN序列分析。这些目标将通过以下具体目标来实现：1. 在其中WRN表达已通过等位基因转移操纵的人细胞系中;在源自敲除小鼠组织的细胞和细胞系中;以及在酵母中，核心将提供： a. 通过细胞遗传学检查分析Werner综合征特征性的总染色体重排的基因组不稳定性，包括整个染色体丢失或获得和杂色易位嵌合体。 B. 在RNA水平评估WRN表达。 C. 用于验证WRN基因突变的DNA序列分析。2. 对于从手术切除获得的肉瘤和脑膜瘤，核心将收集和储存组织用于组织学表征和DNA和RNA分离，用于计划项目，并对配对的正常和肿瘤样本进行WRN DNA序列分析。

DESCRIPTION: (Applicant's Description) The long term goals of Core A, Molecular and Cellular Assays, are [1] to provide genotypic and phenotypic characterization of WRN wild-type, heterozygous, and null human- and mouse-derived cells, cell lines, or tissues generated by this program, and [2] to procure and bank sarcomas and meningiomas for the Program Project in order to perform WRN sequence analysis. These goals will be achieved by the following specific aims: 1. In human cell lines in which WRN expression has been manipulated by allele transfer; in cells and cell lines derived from tissues of knock out mice; and in yeast the Core will provide: a. analysis of genomic instability by cytogenetic examination for gross chromosomal rearrangements characteristic of Werner's syndrome, including whole chromosome loss or gain and variegated translocation mosaicism. b. assessment of WRN expression at the RNA level. c. DNA sequence analysis for verification of WRN gene mutations. 2. For sarcomas and meningiomas obtained from surgical resections, the Core will collect and bank tissue for histologic characterization and DNA and RNA isolation for use in the Program Project and perform WRN DNA sequence analysis of paired normal and tumor samples.