TRANSCRIPTION ACTIVATION AT THE RHABAD OPERON
TRANSCRIPTION ACTIVATION AT THE RHABAD OPERON
批准号:
6386644
负责人:
SUSAN M EGAN
金额:
$10.89万
依托单位国家:
美国
项目类别:
财政年份:
1997
资助国家:
美国
项目状态:
已结题
起止时间:
1997-08-01 至 2003-07-31
关键词:
DNA binding protein DNA directed RNA polymerase Escherichia coli bacterial genetics cytoskeletal proteins gene expression gene induction /repression genetic promoter element genetic transcription molecular cloning mutant nucleic acid sequence operon protein protein interaction protein sequence protein structure function site directed mutagenesis transcription factor
中文摘要
一个转录激活的模型最近被开发出来
英文摘要
A model for transcription activation has recently been developed
based on the structure of E. Coli RNA polymerase, and analysis of
activation by the CRP protein. In this model, the activator protein
directly contacts the RNA polymerase alpha or subunit, resulting in
increased transcription initiation. The long range goal of this
research is to understand the molecular mechanisms of transcription
activation, especially where multiple activator proteins
simultaneously regulate expression of a gene(s). The objective of
this proposal is to investigate the details of transcription activation,
utilizing the E. coli L-rhamnose rhaBAD genes as a model. Full
transcription of rhaBAD requires two activator proteins, RhaS and
CRP. The central hypothesis is that while activator-RNA
polymerase interactions likely contribute to transcription activation,
activator-activator and activator-DNA interactions may also be
important. In order to understand how transcription activation
occurs, it is essential to determine the nature of the protein-protein
and protein-DNA interactions that are required.
Three specific aims will be pursued to accomplish the objectives of
this proposal: 1) characterize DNA binding by RhaS; 2)
characterize transcription activation by RhaS; and 3) characterize
transcription activation by CRP. The role of protein-DNA
interactions will be investigated by isolating mutations which
identify the amino acid-base contacts between RhaS and DNA. To
understand the role of protein-protein interactions in activation,
mutations will be isolated that identify the specific amino acids in
RhaS and CRP that are responsible for transcription activation.
Once activation defective mutations are identified in RhaS and
CRP, second site suppressors will be isolated. The second site
suppressor mutations will identify the protein-protein interactions
that are important for transcription activation. The expectation is
that this research will identify a variety of interactions that
contribute to transcription activation. This work is significant
because it will contribute to a broader understanding of the
mechanisms used for activation, especially with multiple activators.
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The AraC/XylS family activator RhaS negatively autoregulates rhaSR expression by preventing cyclic AMP receptor protein activation.
AraC/XylS 家族激活剂 RhaS 通过阻止环 AMP 受体蛋白激活来负向自动调节 rhaSR 表达。
DOI:
10.1128/jb.00829-08
发表时间:
2010
期刊:
Journal of bacteriology
影响因子:
3.2
作者:
[Wickstrum,JasonR, Skredenske,JeffM, Balasubramaniam,Vinitha, Jones,Kyle, Egan,SusanM]
通讯作者:
Egan,SusanM
Functional consequences of exchanging domains between LacI and PurR are mediated by the intervening linker sequence.
LacI 和 PurR 之间交换结构域的功能后果是由插入的接头序列介导的。
DOI:
10.1002/prot.21412
发表时间:
2007
期刊:
Proteins
影响因子:
2.9
作者:
[Tungtur,Sudheer, Egan,SusanM, Swint-Kruse,Liskin]
通讯作者:
Swint-Kruse,Liskin
Ni+-affinity purification of untagged cAMP receptor protein.
未标记的 cAMP 受体蛋白的 Ni 亲和纯化。
DOI:
10.2144/02334bm01
发表时间:
2002
期刊:
BioTechniques
影响因子:
2.7
作者:
[Wickstrum,JasonR, Egan,SusanM]
通讯作者:
Egan,SusanM
Amino acid contacts between sigma 70 domain 4 and the transcription activators RhaS and RhaR.
西格玛 70 结构域 4 和转录激活剂 RhaS 和 RhaR 之间的氨基酸接触。
DOI:
10.1128/jb.186.18.6277-6285.2004
发表时间:
2004
期刊:
Journal of bacteriology
影响因子:
3.2
作者:
[Wickstrum,JasonR, Egan,SusanM]
通讯作者:
Egan,SusanM
Roles of cyclic AMP receptor protein and the carboxyl-terminal domain of the alpha subunit in transcription activation of the Escherichia coli rhaBAD operon.
环 AMP 受体蛋白和 α 亚基的羧基末端结构域在大肠杆菌 rhaBAD 操纵子转录激活中的作用。
DOI:
10.1128/jb.182.12.3529-3535.2000
发表时间:
2000
期刊:
Journal of bacteriology
影响因子:
3.2
作者:
[Holcroft,CC, Egan,SM]
通讯作者:
Egan,SM
共 6 条
COBRE: U KS: P3: PROTEIN-PROTEIN INTERACTIONS TRANSCRIPTION ACTIVATN BY RHAR
-
批准号:7171175
-
项目类别:
-
资助金额:$6.61万
-
财政年份:2005
-
负责人:SUSAN M EGAN
-
依托单位:
COBRE: U KS: P3: PROTEIN-PROTEIN INTERACTIONS TRANSCRIPTION ACTIVATN BY RHAR
-
批准号:6981853
-
项目类别:
-
资助金额:$10.22万
-
财政年份:2004
-
负责人:SUSAN M EGAN
-
依托单位:
Transcription Activation at the rhaBAD Operon
-
批准号:7457820
-
项目类别:
-
资助金额:$20.06万
-
财政年份:1997
-
负责人:SUSAN M EGAN
-
依托单位:
TRANSCRIPTION ACTIVATION AT THE RHABAD OPERON
-
批准号:6180638
-
项目类别:
-
资助金额:$10.57万
-
财政年份:1997
-
负责人:SUSAN M EGAN
-
依托单位:
TRANSCRIPTION ACTIVATION AT THE RHABAD OPERON
-
批准号:2750129
-
项目类别:
-
资助金额:$9.83万
-
财政年份:1997
-
负责人:SUSAN M EGAN
-
依托单位:
TRANSCRIPTION ACTIVATION AT THE RHABAD OPERON
-
批准号:6019228
-
项目类别:
-
资助金额:$10.26万
-
财政年份:1997
-
负责人:SUSAN M EGAN
-
依托单位:
Transcription Activation at the rhaBAD Operon
-
批准号:6985747
-
项目类别:
-
资助金额:$21.23万
-
财政年份:1997
-
负责人:SUSAN M EGAN
-
依托单位:
Transcription Activation at the rhaBAD Operon
-
批准号:7082051
-
项目类别:
-
资助金额:$20.69万
-
财政年份:1997
-
负责人:SUSAN M EGAN
-
依托单位:
Transcription Activation at the rhaBAD Operon
-
批准号:7243388
-
项目类别:
-
资助金额:$20.07万
-
财政年份:1997
-
负责人:SUSAN M EGAN
-
依托单位:
TRANSCRIPTION ACTIVATION AT THE RHABAD OPERON
-
批准号:2398965
-
项目类别:
-
资助金额:$9.55万
-
财政年份:1997
-
负责人:SUSAN M EGAN
-
依托单位:
FUNCTIONAL COMPARISON OF RHAMNOSE & ARABINOSE OPERONS
-
批准号:2169010
-
项目类别:
-
资助金额:$2.86万
-
财政年份:1993
-
负责人:SUSAN M EGAN
-
依托单位:
FUNCTIONAL COMPARISON OF RHAMNOSE & ARABINOSE OPERONS
-
批准号:3045886
-
项目类别:
-
资助金额:$2.27万
-
财政年份:1992
-
负责人:SUSAN M EGAN
-
依托单位:
FUNCTIONAL COMPARISON OF RHAMNOSE & ARABINOSE OPERONS
-
批准号:3045885
-
项目类别:
-
资助金额:$2.16万
-
财政年份:1991
-
负责人:SUSAN M EGAN
-
依托单位:
海外基金