Utilising the Underlying Genetic Mechanisms of Survival Exhibited by Pseudomonas aeruginosa during Chronic Lung Infection for Novel Drug Target Discov

利用铜绿假单胞菌在慢性肺部感染期间表现出的潜在生存遗传机制来发现新的药物靶点

• 批准号: 1644274
• 金额: --
• 依托单位: University of Birmingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1644274
• 关键词: Utilising; Underlying; Genetic; Mechanisms; Survival

Pseudomonas aeruginosa is a Gram-negative, opportunistic plant and animal pathogen and one of the leading causes of nosocomial infections. As an opportunistic pathogen, P. aeruginosa mainly causes infections in immuno-compromised individuals and its success as a human pathogen can be attributed to its intrinsic resistance to many antibiotics, its adaptability to changes in its environment and its ability to form biofilms.Understanding the underlying genetic mechanisms essential for bacterial survival in the host is key to identifying novel therapeutic targets. Transposon mutagenesis has been shown to be a valuable genetic tool for linking phenotype to genotype by creating mutant libraries with mutations in all non-essential genes. These mutant libraries can then be used to screen for genes essential for growth and survival under a variety of conditions. We aim to construct a transposon mutant library from a chronic infection clinical isolate of Pseudomonas aeruginosa which will subsequently be used to screen for genes essential for survival in the lung during chronic infection. These screens have the potential to identify novel drug targets and/or determine the functions for genes of unknown function.

铜绿假单胞菌是革兰氏阴性的机会性植物和动物病原体，是医院感染的主要原因之一。作为一种机会致病菌，铜绿假单胞菌主要在免疫功能低下的个体中引起感染，其作为人类病原体的成功可归因于其对许多抗生素的内在耐药性，对环境变化的适应性以及形成生物膜的能力。了解细菌在宿主中生存的基本遗传机制是确定新的治疗靶点的关键。转座子突变已被证明是一种有价值的遗传工具，通过在所有非必需基因中创建突变文库，将表型与基因型联系起来。这些突变文库可以用来筛选在各种条件下生长和生存所必需的基因。我们的目标是从慢性感染铜绿假单胞菌临床分离物中构建转座子突变文库，该文库随后将用于筛选慢性感染期间肺部生存所必需的基因。这些筛选有可能识别新的药物靶点和/或确定未知功能基因的功能。