FATE SPECIFICATION ALONG THE SEA URCHIN A-V AXIS

海胆 A-V 轴的命运规范

• 批准号: 6385337
• 负责人: ROBERT C ANGERER
• 金额: $ 39.33万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-07-01 至 2004-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6385337
• 关键词: alternatives to animals in research; cadherins; cellular polarity; developmental genetics; early embryonic stage; gene expression; genetic promoter element; genetic regulatory element; histogenesis; invertebrate embryology; messenger RNA; microinjections; molecular cloning; polymerase chain reaction; protein structure function; receptor expression; regulatory gene; sea urchins; transcription factor

DESCRIPTION: (Adapted from applicant's abstract) This is a proposal to investigate how the animal-vegetal (A-V) axis is determined in the sea urchin embryo. A popular model is that the A-V axis is determined by the interaction of factors emanating from the animal and vegetal regions of the embryo. The Angerer laboratory studies the animal determinants, which are likely to be transcription factors arising from the egg that activate batteries of genes expressed in the animal region of the embryo. There are three objectives. The first objective is to determine whether there is a general mechanism that establishes the animal domain (non-vegetal transcription domain, or NTD) by cloning several factors that reside there. The second objective is to study the interactions between the animal and vegetal factors that establish a border between the domains. The third objective is to assess the role of BMP2/4 signaling in specifying the animal fates.

描述：（改编自申请人的摘要）这是一个建议， 研究如何在海胆中确定动物-植物（A-V）轴 胚胎一个流行的模型是，A-V轴由相互作用决定 来自胚胎的动物和植物区域的因素。的 Angerer实验室研究的是动物的决定因素， 从卵细胞中产生的转录因子，激活一系列基因 在胚胎的动物区域表达。有三个目标。的 第一个目标是确定是否有一个一般机制， 建立动物域（非植物转录域，或NTD）， 克隆几个存在于那里的因子。第二个目标是研究 动物和植物因素之间的相互作用， 域之间。第三个目标是评估BMP 2/4的作用 在指定动物命运时发出信号。