MECHANISM AND FUNCTION OF CONNEXIN INTERACTIONS

连接蛋白相互作用的机制和功能

• 批准号: 6386544
• 负责人: THOMAS H STEINBERG
• 金额: $ 22.31万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6386544
• 关键词: cell cell interaction; electrical property; electrophysiology; gap junctions; membrane channels; membrane permeability; protein structure function; site directed mutagenesis

DESCRIPTION: This application is founded on data showing that expression of connexin45 in cells containing connexin43 alters gap junction communication in those cells. The data suggest that Cx43 and Cx45 interact to form gap junction with unique properties. Three specific aims are proposed. First, the investigator will characterize the interaction between Cx43 and Cx45. Second, he will determine the molecular basis for such interaction mainly through a series of site-directed mutagenesis studies. Third, he will establish the possible significance of these interactions on chemical and electrical coupling.

描述：本申请是建立在数据显示的表达， 含有连接蛋白43的细胞中的连接蛋白45改变间隙连接通讯 在这些细胞中。 提示Cx43和Cx45相互作用形成间隙 具有独特属性的连接。 提出了三个具体目标。 第一、 研究者将描述Cx43和Cx45之间的相互作用。 其次，他将确定这种相互作用的分子基础， 通过一系列定点突变研究。 第三，他将 确定这些相互作用对化学和生物学的可能意义， 电耦合