课题基金 / 基金详情

FOLDING MECHANISMS OF MULTISUBUNIT PEPTIDES AND PROTEINS

FOLDING MECHANISMS OF MULTISUBUNIT PEPTIDES AND PROTEINS
多亚基肽和蛋白质的折叠机制
批准号：
6386635
负责人：
C Robert Matthews
金额：
$28.26万
依托单位：
UNIV OF MASSACHUSETTS MED SCH WORCESTER
依托单位国家：
美国
项目类别：
财政年份：
1996
资助国家：
美国
项目状态：
已结题
起止时间：
1996-07-01 至 2004-06-30
来源：
https://reporter.nih.gov/project-details/6386635
关键词：
acyltransferase carbonate dehydratase circular dichroism conformation fluorescence spectrometry fos protein intermolecular interaction mass spectrometry nuclear magnetic resonance spectroscopy protein denaturation protein folding protein sequence thermodynamics transcription factor

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中文摘要
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英文摘要
This is a renewal proposal (years 5-10) to continue studies of the folding and assembly mechanisms in multisubunit proteins. Specifically, the questions to be addressed are: (1) What are the important structural features of the transient intermediates and intervening transition states observed during the folding of specific representative multimeric protein? (2) Does pre-formed structure in the monomer affect the folding kinetics of the dimer? (3) Do partially folded associated species guide the refolding reaction to the native dimer (trimer)? A combination of biophysical techniques spanning mutagenesis, hydrogen-deuterium exchange, NMR, Mass Spectrometry and rapid-mixing optical techniques will be used to gain information on the time-dependent structure formation and association reactions during the folding of three distinct systems: (a) coiled-coils, (b) the trp-repressor and (c) the trimeric beta-helix proteins.
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