ANGIOTENSIN II RECEPTORS & OTHER MEMBERS OF G PROTEIN LINKED RECEPTOR FAMILY

血管紧张素II(AII)受体，其中有两大类 亚型AT1和AT2属于G蛋白连锁的超家族 受体(Gr)。关于AII的系统发育关系的知识 与这个家族的其他成员的受体可以提供对 这些受体的作用机制和调节。 已取得实质性进展，以阐明这些行动和 AT1受体的机制，但AT2亚型的机制仍然存在 一个谜。我们之前的努力是为了确定系统发育 基于部分氨基酸的AII受体与其他GR的关系 序列，一直是模棱两可的。从另一个角度看问题 AII受体亚型与其他Gr， 这项提案计划检查核酸序列。这 信息可能会导致更明确的系统发育 AII受体亚型的特征。这件事的第二个方面 项目是确定AII上的潜在磷酸化位点 这个项目的受体是确定潜在的磷酸化 AII受体上可能与受体有关的位点 脱敏。AII胞内区的氨基酸 受体将与其他GR受体进行比较 通过磷酸化进行的致密化。

Angiotensin II (AII) receptors, of which there are two major subtypes, AT1, and AT2, belong to a superfamily of G protein-linked receptors (Gr). Knowledge of the phylogenetic relationships of AII receptors with other member of this family can provide insight into the mechanisms of action and regulation of these receptors. Substantial progress has been made to elucidate the actions and mechanisms of the AT1 receptors, but those of the AT2 subtypes remain an enigma. Our previous efforts to determine the phylogenetic relationship of AII receptors to other Gr based on partial amino acid sequences, have been ambiguous. To gain an additional perspective on the phylogenetic relationships of AII receptor subtypes and other Gr, this proposal plans to examine nucleic acid sequences. This information may lead to a more definitive phylogenetic characterization of AII receptor subtypes. A second aspect of this project is to determine potential phosphorylation sites on the AII receptors of this project is to determine potential phosphorylation sites on the AII receptors that may be involved in receptor desensitization. Amino acids in the intracellular domains of AII receptors will be compared with thos of other Gr that undergo densensitization by phosphorylation.