Synthetic Biology and Directed Evolution of Enzymes

合成生物学和酶的定向进化

• 批准号: 1658622
• 金额: --
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1658622
• 关键词: Synthetic; Biology; Directed; Evolution; Enzymes

A considerable amount of work has been applied in finding new enzymes or modifying them to improve some of their useful properties and overcoming the limitations of natural biocatalysis and biotransformation processes. So far, one of the most successful methods to accomplish this is Directed Evolution. This approach includes genetic modification and rational protein engineering to modify enzymes at will, making them more effective in carrying out a process of interest. This is a powerful tool that is usually combined with screening techniques to select the variants of interest out of a large library of mutants. However, to date, screenings are usually laborious, time-consuming, and require intensive work. This PhD proposal will present three different methods for Directed Evolution, which will speed up the screening and selection steps.

大量的工作已经应用于寻找新的酶或对其进行修饰以改善其一些有用的性质，并克服天然生物催化和生物转化过程的局限性。到目前为止，实现这一目标的最成功的方法之一是定向进化。这种方法包括基因修饰和合理的蛋白质工程，以随意修饰酶，使它们更有效地进行感兴趣的过程。这是一个强大的工具，通常与筛选技术相结合，从大型突变体库中选择感兴趣的变体。然而，迄今为止，筛查通常是费力的，耗时的，并且需要密集的工作。这个博士论文将提出三种不同的定向进化方法，这将加快筛选和选择步骤。

项目成果

A primer to directed evolution: current methodologies and future directions.

• DOI: 10.1039/d2cb00231k
• 发表时间: 2023-04-05
• 期刊: RSC CHEMICAL BIOLOGY
• 影响因子: 4.1
• 作者: Selles Vidal, Lara;Isalan, Mark;Heap, John T.;Ledesma-Amaro, Rodrigo
• 通讯作者: Ledesma-Amaro, Rodrigo

Versatile selective evolutionary pressure using synthetic defect in universal metabolism.

• DOI: 10.1038/s41467-021-27266-9
• 发表时间: 2021-11-25
• 期刊: Nature communications
• 影响因子: 16.6
• 作者: Sellés Vidal L;Murray JW;Heap JT
• 通讯作者: Heap JT

A broadly applicable artificial selection system for biomolecule evolution

一种广泛适用的生物分子进化人工选择系统

• 发表时间: 2020
• 作者: Selles Vidal, L