STUDY OF BIOMINERALIZATION MECHANISMS IN DENTIN

牙本质生物矿化机制的研究

• 批准号: 6225759
• 负责人: KOSTAS VERDELIS
• 金额: $ 11.98万
• 依托单位: HOSPITAL FOR SPECIAL SURGERY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2005-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6225759
• 关键词: animal tissue; bioimaging /biomedical imaging; dental visualization; dentinogenesis; electron microscopy; histology; infrared spectrometry; interferometry

This K08 application requests 4 years of support for the Study of Biomineralization Mechanisms in Dentin. Dr. Verdelis received a DDS and a specialty certification in endodontics and has finished required class work for the UNC PhD program. The first 3 years of this award will support the completion of his PhD training, and the last will provide the opportunity to apply for outside funding. The sponsor and primary mentor is Dr. Boskey, Director of Research at HSS, with Dr. Crenshaw at the Dental Research Center of UNC as co-mentor. In the proposed project, a model will be developed for the study of dentinogenesis of bovine incisors using FTIR imaging, electron microscopy, histomorphometry and microanalysis, with respect to the temporal distribution of dentin components during formation. Mantle and peritubular dentin will be compared to intertubular circumpulpal dentin. Knockout mice will also be analyzed to determine the importance of several matrix components. Since the candidate seeks an academic position in endodontics, combining research and clinical activities, this training in biomineralization mechanisms and hard tissue analysis will provide an understanding of the necessary research environment and provide guidance in preparing applications for funding support.

此K08申请申请为期4年的研究支持 牙本质中的生物矿化机制。Verdelis博士收到了DDS和 获得牙髓学专业证书，并已完成必修课程 北卡罗来纳大学博士项目。该奖项的前3年将支持 完成他的博士培训，最后一次将提供机会 申请外部资金。赞助商和主要导师是博斯基博士， HSS研究主任，与克伦肖博士在华盛顿牙科研究中心 北卡罗来纳大学作为共同导师。在拟议的项目中，将为 牛切牙牙本质形成的FTIR成像、电子研究 显微镜、组织形态计量学和显微分析，关于颞叶 牙本质成分在牙本质形成过程中的分布外套膜和肾小管周围 牙本质将被比作管周牙髓间牙本质。基因敲除小鼠会 还要进行分析，以确定几个矩阵成分的重要性。 由于应聘者寻求牙髓学方面的学术职位， 研究和临床活动，这方面的培训生物矿化机制 硬组织分析将提供对必要的 研究环境，并在准备资金申请方面提供指导 支持。