ELEMENTS OF MINERALIZATION MECHANISM IN MANTLE DENTIN

地幔牙本质矿化机制的要素

• 批准号: 6207217
• 负责人: KOSTAS VERDELIS
• 金额: $ 3.09万
• 依托单位: HOSPITAL FOR SPECIAL SURGERY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6207217
• 关键词: animal tissue; collagen; dentin; dentinogenesis; extracellular matrix; gene induction /repression; histochemistry /cytochemistry; infrared spectrometry; intermolecular interaction; lipid metabolism; minerals; molecular assembly /self assembly; normal ossification; phosphoproteins; protein localization; protein protein interaction; proteoglycan; tissue /cell culture; tooth

Dentin formation is largely viewed as the result of interaction of noncollagenous components of the organic matrix with mineral, which leads to the mineral deposition on a collagen scaffold. Although many matrix molecules have been implicated in this process, the necessity of their presence and extent of contribution of each of them to biomineralization remains unknown. The main reason for that is the apparent different rnineralization mechanisms that exist, reflected in dentin by the variation in the composition and properties of organic matrix and the properties of the formed mineral between areas of mantle dentin and circumpulpal dentin. The combination of a high-resolution spectroscopic analytical technique optimized for the analysis of virtually all organic and inorganic mineralization elements with histology provides an excellent means of studying dentin formation. As mantle dentin presents alterations in organic matrix and mineral properties compared to circumpulpal dentin, study of dentin formation in it and comparison to circumpulpal dentin is expected to further help establish the mineralization pathways. For this purpose, the development of a model for study of mantle dentin formation on bovine teeth is suggested. By applying the same methods to dentin from mice that are knockout for a specific matrix macromolecule (at the present time osteopontin, bone sialoprotein, osteonectin, biglycan and decorin) the necessity of each macromolecule for proper dentin formation will be assessed.

牙本质的形成在很大程度上被认为是有机基质中非胶原性成分与矿物质相互作用的结果，从而导致矿物质沉积在胶原支架上。尽管许多基质分子参与了这一过程，但它们存在的必要性以及它们对生物矿化的贡献程度仍不清楚。其主要原因是牙本质中存在明显不同的矿化机制，反映在牙本质中的有机基质的组成和性质以及形成的矿物性质的不同，反映在牙本质的套层和牙髓周围。针对几乎所有有机和无机矿化元素的分析而优化的高分辨率光谱分析技术与组织学的结合为研究牙本质的形成提供了一种极好的手段。由于与牙髓周围牙本质相比，盖层牙本质的有机基质和矿物质性质发生了变化，因此研究它的牙本质形成并与牙髓周围牙本质进行比较将有助于进一步确定其矿化途径。为此，建议建立一种研究牛牙外套膜牙本质形成的模型。通过将相同的方法应用于小鼠的牙本质，这些大分子被特定的基质大分子(目前的骨桥蛋白、骨涎蛋白、骨连接蛋白、双聚糖和核心蛋白)敲除，将评估每个大分子正确形成牙本质的必要性。