Elucidating the role of the AP2 endocytic adaptor complex on C.albicans virulence.

阐明 AP2 内吞接头复合物对白色念珠菌毒力的作用。

• 批准号: 1660283
• 金额: --
• 依托单位: University of Sheffield
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1660283
• 关键词: Elucidating; role; AP2; endocytic; adaptor

We have deleted an essential subunit of the AP2 endocytic adaptor complex in Candida albicans, and observed marked defects in polarised growth. As shown previously, the apm4 deletion mutant is unable to form long, thin filamentous cells under inducing conditions, instead growing wide and misshapen hyphae. The mutant is only slightly impaired in fluid phase endocytosis, leading us to hypothesise that AP2 plays a role in the endocytosis of specific cargoes which are important to polarised growth. It has recently become clear that a balance of endocytosis and secretion is essential for the highly polarised growth seen in filamentous fungi, however many of the key players remain unknown (Schultzhaus and Shaw, 2017). This polarised growth is a key aspect of cell biology, and is also central to Candida albicans virulence (Mayer et al., 2013). Therefore, elucidating the role of the AP2 endocytic adaptor complex in hyphal growth and virulence of Candida albicans is of great importance.

我们已经删除了一个重要的亚基的AP2内吞适配器复合物在白色念珠菌，并观察到显着的缺陷极化生长。如前所述，apm4缺失突变体在诱导条件下不能形成细长的丝状细胞，而是生长宽而畸形的菌丝。突变体在液相内吞作用中仅轻微受损，这使我们假设AP 2在对极化生长重要的特定货物的内吞作用中起作用。最近已经清楚的是，内吞作用和分泌的平衡对于丝状真菌中观察到的高度极化生长至关重要，然而许多关键参与者仍然未知（Schultzhaus和Shaw，2017）。这种极化生长是细胞生物学的一个关键方面，也是白色念珠菌毒力的核心（Mayer et al.，2013年）。因此，阐明AP 2内吞接头复合物在白念珠菌菌丝生长和毒力中的作用具有重要意义。

项目成果

AP-2-Dependent Endocytic Recycling of the Chitin Synthase Chs3 Regulates Polarized Growth in Candida albicans

• DOI: 10.1128/mbio.02421-18
• 发表时间: 2019-03-01
• 期刊: MBIO
• 影响因子: 6.4
• 作者: Knafler, H. C.;Smaczynska-de Rooij, I. I.;Ayscough, K. R.
• 通讯作者: Ayscough, K. R.