BRAIN OXYGENATION AND METABOLISM

脑氧合和新陈代谢

• 批准号: 6343592
• 负责人: Anna Pastuszko
• 金额: $ 34.87万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-01-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6343592
• 关键词: amine oxidase (flavin); blood chemistry; blood flow measurement; brain circulation; brain disorders; brain metabolism; cerebral ischemia /hypoxia; dopamine; free radicals; heart /lung bypass; high performance liquid chromatography; hydroxyl radical; hypoxia; in situ hybridization; medical complication; microdialysis; oxygen tension; oxygen transport; postoperative complications; radioimmunoassay; retina circulation; retina disorder; stress proteins; swine

A team of investigators, surgical specialists from the Departments of Anesthesiology and Surgery and basic researchers from the Department of Biochemistry & Biophysics, will critically evaluate brain oxygenation and metabolism during and after low flow hypothermic cardiopulmonary bypass (LFCPB) and deep hypothermic circulatory arrest (DHCA). These studies will increase our understanding of the cellular and molecular mechanisms responsible for a brain and retinal dysfunction resulting from cardiopulmonary bypass (CPB) so that effective strategies can be developed for protecting the brain. Specifically, the following hypotheses will be tested: 1. During cardiopulmonary bypass there is a metabolically important deficiency in the oxygen pressure in the brain and retina of newborn piglets. This oxygen deficiency will result in: a/. increases in serum S-100 protein. b/. increases in the extracellular level of dopamine. c/. Increase generation of hydroxyl radicals. d/. induction of expression of heat shock protein. 2. Increases in the extracellular level of dopamine during bypass contributes to development of disturbance of cellular metabolism and cell function through: a/. Generation of free radicals during spontaneous autooxidation of the excess dopamine an during its enzymatic oxidation of monoamine oxidase. b/. Causing alterations in the sensitivity of the D1-like and D2-like receptors by modifying the Kd for agonists and/or the number of receptors (Bma). c/. Causing vasoconstriction which in turn is responsible for post-bypass hypoperfusion. 3. Polyethylene glycol modified hemoglobin (PEG) can provide significant improvements in oxygen delivery and protection from the metabolic disturbances of CPB.

一组调查人员，来自科室的外科专家