BRAIN OXYGENATION AND METABOLISM
BRAIN OXYGENATION AND METABOLISM
批准号:
6343592
负责人:
Anna Pastuszko
金额:
$34.87万
依托单位国家:
美国
项目类别:
财政年份:
1999
资助国家:
美国
项目状态:
已结题
起止时间:
1999-01-01 至 2002-12-31
关键词:
amine oxidase (flavin) blood chemistry blood flow measurement brain circulation brain disorders brain metabolism cerebral ischemia /hypoxia dopamine free radicals heart /lung bypass high performance liquid chromatography hydroxyl radical hypoxia in situ hybridization medical complication microdialysis oxygen tension oxygen transport postoperative complications radioimmunoassay retina circulation retina disorder stress proteins swine
中文摘要
一支由调查人员、外科专家组成的团队
麻省理工学院麻醉学和外科基础研究人员
生物化学和生物物理学,将批判性地评估脑氧合作用和
低流量低温体外循环期间和术后的代谢
(LFCPB)和深低温停循环(DHCA)。这些研究将
加深我们对细胞和分子机制的理解
导致大脑和视网膜功能障碍的原因
体外循环(CPB),以便制定有效的策略
为了保护大脑。
具体地说,将检验以下假设:
1.在体外循环期间,有一种代谢上重要的
新生儿脑和视网膜氧分压缺乏
小猪。
这种缺氧症会导致:
A/.血清S-100蛋白升高。
B/。细胞外多巴胺水平的增加。
C/.增加羟基自由基的生成。
D/.热休克蛋白的诱导表达。
2.体外循环期间细胞外多巴胺水平升高
有助于发展细胞代谢紊乱和细胞
功能通过:
A/.细胞自发自氧化过程中自由基的产生
在单胺氧化酶的酶促氧化过程中过量的多巴胺。
B/。导致类D_1和类D_2的灵敏度改变
通过改变受体激动剂的Kd和/或受体的数量
(BMA)。
C/.导致血管收缩,进而导致搭桥后
低灌注率。
3.聚乙二醇化血红蛋白(PEG)可提供显著的
改善氧气输送和防止新陈代谢
CPB的骚乱。
英文摘要
A team of investigators, surgical specialists from the Departments of
Anesthesiology and Surgery and basic researchers from the Department of
Biochemistry & Biophysics, will critically evaluate brain oxygenation and
metabolism during and after low flow hypothermic cardiopulmonary bypass
(LFCPB) and deep hypothermic circulatory arrest (DHCA). These studies will
increase our understanding of the cellular and molecular mechanisms
responsible for a brain and retinal dysfunction resulting from
cardiopulmonary bypass (CPB) so that effective strategies can be developed
for protecting the brain.
Specifically, the following hypotheses will be tested:
1. During cardiopulmonary bypass there is a metabolically important
deficiency in the oxygen pressure in the brain and retina of newborn
piglets.
This oxygen deficiency will result in:
a/. increases in serum S-100 protein.
b/. increases in the extracellular level of dopamine.
c/. Increase generation of hydroxyl radicals.
d/. induction of expression of heat shock protein.
2. Increases in the extracellular level of dopamine during bypass
contributes to development of disturbance of cellular metabolism and cell
function through:
a/. Generation of free radicals during spontaneous autooxidation of the
excess dopamine an during its enzymatic oxidation of monoamine oxidase.
b/. Causing alterations in the sensitivity of the D1-like and D2-like
receptors by modifying the Kd for agonists and/or the number of receptors
(Bma).
c/. Causing vasoconstriction which in turn is responsible for post-bypass
hypoperfusion.
3. Polyethylene glycol modified hemoglobin (PEG) can provide significant
improvements in oxygen delivery and protection from the metabolic
disturbances of CPB.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Newborn brain oxygenation and cellular injury
-
批准号:6611375
-
项目类别:
-
资助金额:$32.1万
-
财政年份:2002
-
负责人:Anna Pastuszko
-
依托单位:
Newborn brain oxygenation and cellular injury
-
批准号:6901932
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项目类别:
-
资助金额:$32.1万
-
财政年份:2002
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负责人:Anna Pastuszko
-
依托单位:
Newborn brain oxygenation and cellular injury
-
批准号:6513861
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项目类别:
-
资助金额:$32.1万
-
财政年份:2002
-
负责人:Anna Pastuszko
-
依托单位:
Newborn brain oxygenation and cellular injury
-
批准号:6790603
-
项目类别:
-
资助金额:$32.1万
-
财政年份:2002
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负责人:Anna Pastuszko
-
依托单位:
Brain Oxygen and Metabolism in Cardiopulmonary Bypass
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批准号:7009618
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项目类别:
-
资助金额:$36.5万
-
财政年份:1999
-
负责人:Anna Pastuszko
-
依托单位:
Brain metabolism and neuroprotection in cardiopulmonary bypass
-
批准号:7924600
-
项目类别:
-
资助金额:$45.91万
-
财政年份:1999
-
负责人:Anna Pastuszko
-
依托单位:
BRAIN OXYGENATION AND METABOLISM
-
批准号:2757918
-
项目类别:
-
资助金额:$33.13万
-
财政年份:1999
-
负责人:Anna Pastuszko
-
依托单位:
Brain Oxygen and Metabolism in Cardiopulmonary Bypass
-
批准号:6879558
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项目类别:
-
资助金额:$37.4万
-
财政年份:1999
-
负责人:Anna Pastuszko
-
依托单位:
Brain Oxygen and Metabolism in Cardiopulmonary Bypass
-
批准号:7184343
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项目类别:
-
资助金额:$37.57万
-
财政年份:1999
-
负责人:Anna Pastuszko
-
依托单位:
Brain metabolism and neuroprotection in cardiopulmonary bypass
-
批准号:8280214
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项目类别:
-
资助金额:$45.47万
-
财政年份:1999
-
负责人:Anna Pastuszko
-
依托单位:
Brain metabolism and neuroprotection in cardiopulmonary bypass
-
批准号:8072635
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项目类别:
-
资助金额:$45.47万
-
财政年份:1999
-
负责人:Anna Pastuszko
-
依托单位:
BRAIN OXYGENATION AND METABOLISM
-
批准号:6490590
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项目类别:
-
资助金额:$35.72万
-
财政年份:1999
-
负责人:Anna Pastuszko
-
依托单位:
Brain Oxygen and Metabolism in Cardiopulmonary Bypass
-
批准号:6779316
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项目类别:
-
资助金额:$38.88万
-
财政年份:1999
-
负责人:Anna Pastuszko
-
依托单位:
BRAIN OXYGENATION AND METABOLISM
-
批准号:6139254
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项目类别:
-
资助金额:$34.04万
-
财政年份:1999
-
负责人:Anna Pastuszko
-
依托单位:
Brain metabolism and neuroprotection in cardiopulmonary bypass
-
批准号:7730099
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项目类别:
-
资助金额:$46.76万
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财政年份:1999
-
负责人:Anna Pastuszko
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依托单位:
BIOLOGICAL ROLE OF NEUROCATIN--NOVEL BRAIN PEPTIDE
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批准号:3413857
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项目类别:
-
资助金额:$11.41万
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财政年份:1990
-
负责人:Anna Pastuszko
-
依托单位:
BIOLOGICAL ROLE OF NEUROCATIN--NOVEL BRAIN PEPTIDE
-
批准号:3413859
-
项目类别:
-
资助金额:$12.43万
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财政年份:1990
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负责人:Anna Pastuszko
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依托单位:
BIOLOGICAL ROLE OF NEUROCATIN--NOVEL BRAIN PEPTIDE
-
批准号:3413860
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项目类别:
-
资助金额:$12.68万
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财政年份:1990
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负责人:Anna Pastuszko
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依托单位:
海外基金