INTERVENTION IN ATHEROGENESIS BY 7ALPHA HYDROXYLASE

描述：申请人建议确定是否增加 7 α-羟化酶活性在肝脏或异位表达 巨噬细胞中的7 α-羟化酶对动脉粥样硬化的形成有有利的作用， 高脂血症小鼠 第一个目标是确定转基因如何 7 α-羟化酶在高脂血症小鼠肝脏中的表达 影响血浆脂蛋白水平和动脉粥样硬化形成。 第二个目标是 为了确定7 α-羟化酶的转基因表达是否在 高脂血症小鼠巨噬细胞对动脉粥样硬化形成的影响。 换句 研究表明，高脂血症小鼠的骨髓将被干细胞取代， 从表达巨噬细胞特异性7 α-羟化酶的小鼠获得 转基因。

DESCRIPTION: The applicant proposes to determine if augmentation of 7alpha-hydroxylase activity in the liver or ectopic expression of 7alpha-hydroxylase in macrophages has a favorable effect on atherogenesis in hyperlipidemic mice. The first aim is to determine how transgenic expression of 7alpha-hydroxylase in the liver of hyperlipidemic mice influences plasma lipoprotein levels and atherogenesis. The second aim is to determine if the transgenic expression of 7alpha-hydroxylase in macrophages of hyperlipidemic mice influences atherogenesis. In other studies, bone marrow of hyperlipidemic mice will be replaced with stem cells obtained from mice expressing a 7alpha-hydroxylase macrophage-specific transgene.