INTERVENTION IN ATHEROGENESIS BY 7ALPHA HYDROXYLASE

7α 羟化酶干预动脉粥样硬化

• 批准号: 6389640
• 负责人: ROGER A DAVIS
• 金额: $ 32.06万
• 依托单位: SAN DIEGO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-05-01 至 2002-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6389640
• 关键词: atherosclerosis; blood lipoprotein; bone marrow transplantation; enzyme activity; enzyme induction /repression; gene therapy; genetically modified animals; hyperlipidemia; laboratory mouse; liver cells; liver metabolism; macrophage; method development; nonhuman therapy evaluation; steroid 7alpha hydroxylase; tissue /cell culture

DESCRIPTION: The applicant proposes to determine if augmentation of 7alpha-hydroxylase activity in the liver or ectopic expression of 7alpha-hydroxylase in macrophages has a favorable effect on atherogenesis in hyperlipidemic mice. The first aim is to determine how transgenic expression of 7alpha-hydroxylase in the liver of hyperlipidemic mice influences plasma lipoprotein levels and atherogenesis. The second aim is to determine if the transgenic expression of 7alpha-hydroxylase in macrophages of hyperlipidemic mice influences atherogenesis. In other studies, bone marrow of hyperlipidemic mice will be replaced with stem cells obtained from mice expressing a 7alpha-hydroxylase macrophage-specific transgene.

描述：申请人建议确定是否增加 肝脏中7 α-羟化酶活性或异位表达 巨噬细胞中的7 α-羟化酶对动脉粥样硬化的形成有有利的作用， 高脂血症小鼠 第一个目标是确定转基因如何 7 α-羟化酶在高脂血症小鼠肝脏中的表达 影响血浆脂蛋白水平和动脉粥样硬化形成。 第二个目标是 为了确定7 α-羟化酶的转基因表达是否在 高脂血症小鼠巨噬细胞对动脉粥样硬化形成的影响。 换句 研究表明，高脂血症小鼠的骨髓将被干细胞替代， 从表达巨噬细胞特异性7 α-羟化酶的小鼠获得 转基因。